International Journal of Cell Cloning, Vol 1, 401-411, Copyright © 1983 by AlphaMed Press
Myelopoiesis of human bone marrow cells in a micro-agar culture system: comparison of two sources of colony stimulating activity (CSA)
G Konwalinka, C Peschel, D Geissler, J Boyd, B Tomaschek, R Odavic and H Braunsteiner
Human bone marrow cells were grown in a micro-agar culture system in the
presence of human placenta (HPCM) and giant cell tumor conditioned media
(GCT). The effects of HPCM and GCT conditioned media on linearity, growth
dynamics, and morphological composition of colonies were studied after 7
and 14 days of incubation. Under the described conditions the dose-response
curves for HPCM and GCT were different: on day 7 maximal stimulation was
obtained with 2.5% HPCM and 20% GCT; on day 14 a maximal response was
reached with 1.25% HPCM and 5% GCT. Both stimuli produced maximal growth
after 7 days of incubation, followed by a rapid decrease in the number of
formed colonies up to day 14. The morphological study of aggregates showed
that after 7 days of incubation 80% pure granulocyte and 20% mixed
granulocyte-macrophage colonies were found in the presence of both stimuli.
However, on day 14 the incidence of granulocyte-macrophage colonies
increased to 60%, whereas the percentage of pure granulocyte colonies
decreased to 20%. The frequency of eosinophil colonies was relatively low
(median 15%) with both stimuli. The described system can be applied
successfully for studies of myelopoiesis in vitro. Both sources of colony
stimulating activitites (CSA) employed had no significant difference in
their ability to stimulate myelopoiesis.
