International Journal of Cell Cloning, Vol 1, 451-463, Copyright © 1983 by AlphaMed Press
The influence of choline chloride on murine hematopoiesis in vivo and in vitro
VS Gallicchio, MG Chen, C Gamba-Vitalo and TD Watts
We report here studies to further document the ability of cholinergic
agonists to influence murine hematopoiesis. B6C3F1 mice were administered
ultrapure choline (0.14, 1.4, and 7.0 mg/animal) i.p. for three days, then
randomly sacrificed for assessment of hematological parameters. No
significant change was observed in the packed red cell volume; however, the
WBC was elevated significantly (85-400%) over noncholine administered
controls during all days examined [1-12]. In vitro choline (10 mM)
increased heterogeneous marrow-derived CFU-GM colony formation 210% above
controls. Nonadherent marrow cell-derived CFU-GM produced a significant
elevation in colony formation (36%), suggesting choline may influence
directly a portion of the CFU-GM population. A twofold increase in the
CFU-GM kill after 16 mM hydroxyurea exposure suggests choline can stimulate
a subpopulation of CFU-GM not normally in cell cycle. In addition, choline
(10 mM) was effective in stimulating CSF activity from marrow-derived
conditioned medium. Dose-response studies demonstrated that choline was
also stimulatory for megakaryocyte colony formation (CFU-Mk), 26% at 5 mM.
Erythroid precursor stem cells, CFU-E/BFU-E, were reduced significantly
(10-50%) at all choline concentrations tested (1-50 mM). These studies
document the ability of choline to influence hematopoiesis, and provide
further evidence that cholinergic mechanisms may be important in the
control of steady-state hematopoiesis.
