Stem Cells http://www.epitomics.com
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ratajczak, M. Z.
Right arrow Articles by Gewirtz, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ratajczak, M. Z.
Right arrow Articles by Gewirtz, A. M.

Stem Cells, Vol 12, 599-603, Copyright © 1994 by AlphaMed Press

ORIGINAL ARTICLES

Growth factor stimulation of cryopreserved CD34+ bone marrow cells intended for transplant: an in vitro study to determine optimal timing of exposure to early acting cytokines

MZ Ratajczak, J Ratajczak, DA Kregenow and AM Gewirtz
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia.

Stimulating CD34+ hematopoietic cells with growth factors prior to transplantation may decrease the duration of post-transplant aplasia. The optimal time in which to deliver this stimulus remains unclear. We therefore sought to determine if progenitor cell cloning efficiency, as reflected by colony forming units-granulocyte-macrophage (CFU-GM) colony formation, differed when growth factor stimulation was carried out prior to freezing or after thawing. To address this issue, CD34+ marrow cells were suspended in Iscove's medium with 20% bovine calf serum. They were then stimulated with kit ligand (KL), interleukin-3 (IL-3), and interleukin-1 beta (IL-1 beta) for 36 h either prior to cryopreservation or immediately after thawing. We observed that cells stimulated prior to freezing formed more CFU-GM colonies than cells stimulated after thawing. We also found that CD34+ cells stimulated with growth factors either before or after freezing gave rise to more CFU-GM colonies than thawed cells plated without prestimulation. Thus, the cloning efficiency of stored marrow, as reflected by CFU-GM colony formation, may be effectively enhanced by pretreating cells with hematopoietic growth factors. Our data further suggest that the optimal time for this treatment is before cryopreservation as opposed to immediately after thawing.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 BloodHome page
M. Z. Ratajczak, J. Ratajczak, B. Machalinski, R. Mick, and A. M. Gewirtz
In Vitro and In Vivo Evidence That Ex Vivo Cytokine Priming of Donor Marrow Cells May Ameliorate Posttransplant Thrombocytopenia
Blood, January 1, 1998; 91(1): 353 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
M. Ratajczak, J Ratajczak, J Ford, R Kregenow, W Marlicz, and A. Gewirtz
FLT3/FLK-2 (STK-1) Ligand does not stimulate human megakaryopoiesis in vitro
Stem Cells, January 1, 1996; 14(1): 146 - 150.
[Abstract]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.stemcellsportal.com/
Copyright © 1994 by AlphaMed Press.