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Stem Cells, Vol 13, 607-612, Copyright © 1995 by AlphaMed Press

REVIEWS

Serum-free culture of hematopoietic stem cells: a review

JS Lebkowski, LR Schain and TB Okarma
Applied Immune Sciences, Inc., Santa Clara, California 95054-1114, USA.

The development of serum-free systems for the maintenance and expansion of both primitive and committed hematopoietic progenitors has numerous applications in both basic and clinical research. Many different media have been tested and refined over the years, and current formulations now yield results similar to those observed with fetal bovine serum- based medias. Using these serum-free culture systems, the impact of the cell microenvironment and individual growth factors on primitive and maturing stem cells have both been studied. The utility of progenitor populations expanded ex vivo under serum-free conditions is under investigation.


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Copyright © 1995 by AlphaMed Press.