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Overexpression of Granulocyte Colony-Stimulating Factor In Vivo Decreases the Level of Polyploidization of Mouse Bone Marrow Megakaryocytes

^a Department of Pathology, National Children's Medical Research Center, Tokyo, Japan;
^b Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan;
^c Department of Pathology, Keio University School of Medicine, Tokyo, Japan

Key Words. G-CSF • TPO • Megakaryocyte • Megakaryocytopoiesis • DNA • Ploidy

Dr. Junichiro Fujimoto, Department of Pathology, National Children's Medical Research Center 3-35-31, Taishido, Setagaya-ku, Tokyo, 154, Japan.

The in vivo effect of G-CSF on the maturation of mouse bone marrow megakaryocytes was studied by monitoring the DNA contents. Megakaryocytes were first identified by a specific 1C2 monoclonal antibody against mouse platelets and megakaryocytes and DNA contents of these cells were measured by propidium iodine. Megakaryocytes of mice transgenic for human G-CSF had a modal DNA class of 8N, showing a striking contrast to the previous reports that normal mouse megakaryocytes from most strains have 16N DNA content as a modal class. Daily 10 µg administration of G-CSF to mice for three to five days affected the DNA distribution pattern of bone marrow megakaryocytes, with a higher proportion of cells having 8N DNA contents. This G-CSF treatment, however, did not influence the peripheral blood platelet count or bone marrow megakaryocyte number. Administration of G-CSF along with thrombopoietin (TPO) reduced the proportion of megakaryocytes, with 32N DNA, the DNA class that was increased by TPO. Finally, the presence of mRNA for the mouse G-CSF receptor was demonstrated in two megakaryoblastic cell lines by reverse transcriptase polymerase chain reaction. These results indicated that G-CSF may have a suppressive effect on the maturation of mouse bone marrow megakaryocytes when monitored by the DNA polyploidy. Although further study is clearly necessary, the presence of mRNA for the G-CSF receptor in megakaryocytic lineage strongly suggests the direct action of G-CSF on this cell lineage.

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