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Human Growth Hormone Antagonist (G120R) Delivered by a Murine Yolk Sac Cell-Derived Mini-Organ Decreases the Growth Rate of Mice

Edison Biotechnology Institute, Molecular and Cellular Biology Program and Department of Biological Sciences of Ohio University, Athens, Ohio, USA

Key Words. Mini-organ • G120R • Yolk sac cell • IGF-1 • Mice • Gene delivery

Dr. Thomas E. Wagner, Edison Biotechnology Institute of Ohio University, Athens, OH 45701, USA.

Long-term cultured murine embryonic yolk sac cells that are capable of forming capillary structures when cultured on base membrane proteins (Matrigel) were successfully transfected with a human growth hormone antagonist (G120R) gene. Cells that stably express relatively high levels of G120R were co-implanted s.c. with Matrigel into BALB/c mice. G120R can be detected in the sera of those implanted mice for more than 14 days at levels from 4 ng/ml to 28 ng/ml. The insulin-like growth factor-1 levels in the sera of those implanted mice were significantly affected by the delivered G120R. One of the physiological effects of G120R delivered by this murine embryonic yolk sac cell-derived mini-organ system is to decrease the growth rate of the implanted mice. This gene delivery system can also be used as an alternative to transgenic animals to study protein function in vivo.