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Difference between Expression of Adhesion Molecules on CD34⁺ Cells from Bone Marrow and G-CSF-Stimulated Peripheral Blood

^a Bone Marrow Transplantation Unit,
^b Department of Oncology and Hematology, University Hospital Hamburg, Hamburg, Germany

Key Words. Adhesion molecules • Mobilization of CD34⁺ cells • Bone marrow

Dr. Nicolaus Kröger, Bone Marrow Transplantation Unit, Department of Oncology and Hematology, University Hospital Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany.

Three-color immunofluorescence cytometry was used to quantify the expression of different adhesion molecules on CD34⁺ cells of steady-state bone marrow (BM) and peripheral blood stem cells (PBSC) after mobilizing with G-CSF (10 µg/kg/body weight) in nine cancer patients undergoing high-dose chemotherapy with subsequent autologous blood stem cell rescue. The expression rate of each adhesion molecule on CD34⁺ cells showed great inter-individual variations. High expression (>50%) on CD34⁺ cells from PBSC and BM was found for CD58 (leukocyte function-associated antigen-3), CD31 (platelet-endothelial cell adhesion molecule-1), CD11a (leukocyte function-associated antigen-1) and CD49d (very late activation antigen-4); a moderate expression (20%-40%) was seen for CD49e (very late activation antigen-5), CD62L (leukocyte-endothelial cell adhesion molecule), CD54 (ICAM-1) and CD117 (c-kit).

c-kit, CD58, CD62L and CD49d were less expressed on CD34⁺ cells of PBSC than of BM, the difference being statistically significant for CD49d (p < 0.05). CD49e and CD37 were expressed more in PBSC than BM without being statistically significant. The mean fluorescence intensity for all adhesion molecules on CD34⁺ cells did not differ significantly between PBSC and BM. The significantly lower expression of CD49d on G-CSF-mobilized PBSCs might suggest that downregulation of this molecule may be involved in the process of peripheral stem cell mobilization.

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