HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sugiura, K. Articles by Ikehara, S. Search for Related Content PubMed PubMed Citation Articles by Sugiura, K. Articles by Ikehara, S.

Characterization of Natural Suppressor Cells in Human Bone Marrow

^a First Department of Pathology, Kansai Medical University, Moriguchi-City, Osaka, Japan;
^b Department of Pediatrics, North Shore University Hospital-New York University School of Medicine, Manhasset, New York, USA;
^c First Department of Internal Medicine, Kansai Medical University, Moriguchi-City, Osaka, Japan;
^d Department of Pediatrics, All Children's Hospital, University of South Florida, St. Petersburg, Florida, USA;
^e Department of Pediatrics, Schneider Children's Hospital, Long Island Jewish Medical Center, New Hyde Park, New York, USA;
^f Novartis Pharma K.K. Takarazuka-City, Hyogo, Japan

Key Words. Natural suppressor • CD34⁺33⁺ cells • Myeloid progenitors

Dr. Susumu Ikehara, First Department of Pathology, Kansai Medical University, 10-15 Fumizono, Moriguchi-City, Osaka 570, Japan.

Natural suppressor (NS) cells, which exert nonspecific suppressive activity in an unprimed manner, have been found in mouse, rabbit and monkey bone marrow (BM). In the present study, we characterize NS cells in human BM. NS activity was found in a fraction of low density (1.055-1.065 g/ml) BM cells that had been depleted of T cells, B cells, and monocytes. The NS activity was significantly decreased by the depletion of CD34⁺ or CD33⁺ cells but not CD56⁺ cells. The NS activity was indeed detected in isolated CD34⁺ cells and further enriched in CD34⁺CD33⁺ cells. Hematopoietic progenitor cells committed to the myeloid lineage were also enriched in the CD34⁺CD33⁺ cells, which significantly correlated to the NS activity. From these findings, it is strongly suggested that NS activity in human BM is exerted by the myeloid hematopoietic progenitors. Since cell-to-cell contact was not necessary for the action, NS cells seemed to secrete soluble mediator(s). Transforming growth factor-ß1 and leukemia inhibitory factor were, however, not the candidates, based on experiments using neutralizing antibodies.

This article has been cited by other articles:

				K. Sugiura, H. Hisha, J. Ishikawa, Y. Adachi, S. Taketani, S. Lee, T. Nagahama, and S. Ikehara Major Histocompatibility Complex Restriction Between Hematopoietic Stem Cells and Stromal Cells In Vitro Stem Cells, January 1, 2001; 19(1): 46 - 58. [Abstract] [Full Text]