HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Patchen, M.L. Articles by Quesenberry, P.J. Search for Related Content PubMed PubMed Citation Articles by Patchen, M.L. Articles by Quesenberry, P.J.

Mobilization of Peripheral Blood Progenitor Cells by Betafectin^® PGG-Glucan Alone and in Combination with Granulocyte Colony-Stimulating Factor

Alpha-Beta Technology, Inc., Worcester, Massachusetts, USA and
^a The University of Massachusetts Cancer Center, Worcester, Massachusetts, USA

Key Words. Hematopoiesis • Peripheral blood progenitor cells • Mobilization • Reconstitution • Radioprotection • Chemokines • PGG-Glucan • Betafectin^® • G-CSF

Dr. Myra L. Patchen, Preclinical Pharmacology, Alpha-Beta Technology, Inc., One Innovation Drive, Worcester, MA 01605 USA.

Betafectin^® PGG-Glucan, a novel ß-(1,6) branched ß-(1,3) glucan purified from the cell walls of Saccharomyces cerevisiae, has been shown to synergize with myeloid growth factors in vitro and to enhance hematopoietic recovery in myelosuppressed mice and primates. Here we report that PGG-Glucan is also capable of mobilizing peripheral blood progenitor cells (PBPC). PGG-Glucan (0.5 mg/kg to 16 mg/kg) was administered intravenously to C3H/HeN male mice and blood collected at times ranging from 30 min to seven days after injection. Based on granulocyte-macrophage colony-forming cell (GM-CFC) levels, peak mobilization occurred 30 min after a 2 mg/kg PGG-Glucan dose. At this time GM-CFC numbers in PGG-Glucan-treated mice were approximately fourfold greater than in saline-treated control mice. A second, smaller wave of GM-CFC mobilization (approximately twofold increase) also occurred on days 4 and 5 after PGG-Glucan treatment. Mobilization was not associated with the induction of -chemokines, which have recently been reported to induce rapid progenitor cell mobilization. Competitive repopulation experiments performed in irradiated female C3H/HeN mice revealed that, at three months after transplantation, more male DNA was present in bone marrow, splenic, and thymic tissues from animals transplanted with cells obtained from mice 30 min after a 2 mg/kg PGG-Glucan dose than in tissues from animals transplanted with cells obtained from saline-treated mice. Additional experiments evaluated the mobilization effects of PGG-Glucan (2 mg/kg) administered to mice which had been pretreated for three consecutive days with G-CSF (125 µg/kg/day). When blood was collected 30 min after PGG-Glucan treatment, the number of GM-CFC mobilized in combination-treated mice was additive between the number mobilized in mice treated with G-CSF alone and the number mobilized in mice treated with PGG-Glucan alone. These studies demonstrate that: A) PGG-Glucan can rapidly mobilize PBPC; B) the kinetic pattern of PGG-Glucan-induced mobilization is different from that of the CSFs; C) the reconstitutional potential of PGG-Glucan mobilized cells is greater than that of steady-state PBPC, and D) PGG-Glucan can enhance G-CSF-mediated PBPC mobilization.

This article has been cited by other articles:

				D. E. Cramer, S. Wagner, B. Li, J. Liu, R. Hansen, R. Reca, W. Wu, E. Z. Surma, D. A. Laber, M. Z. Ratajczak, et al. Mobilization of Hematopoietic Progenitor Cells by Yeast-Derived {beta}-Glucan Requires Activation of Matrix Metalloproteinase-9 Stem Cells, May 1, 2008; 26(5): 1231 - 1240. [Abstract] [Full Text] [PDF]

				H. Lin, S. W. Y. Cheung, M. Nesin, B. R. Cassileth, and S. Cunningham-Rundles Enhancement of Umbilical Cord Blood Cell Hematopoiesis by Maitake Beta-Glucan Is Mediated by Granulocyte Colony-Stimulating Factor Production Clin. Vaccine Immunol., January 1, 2007; 14(1): 21 - 27. [Abstract] [Full Text] [PDF]

				B. W. LeBlanc, J. E. Albina, and J. S. Reichner The effect of PGG-{beta}-glucan on neutrophil chemotaxis in vivo J. Leukoc. Biol., April 1, 2006; 79(4): 667 - 675. [Abstract] [Full Text] [PDF]

				E. A. Sweeney, G. V. Priestley, B. Nakamoto, R. G. Collins, A. L. Beaudet, and T. Papayannopoulou Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence PNAS, June 6, 2000; 97(12): 6544 - 6549. [Abstract] [Full Text] [PDF]