HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Desplat, V. Articles by Denizot, Y. Search for Related Content PubMed PubMed Citation Articles by Desplat, V. Articles by Denizot, Y.

Expression of Platelet-Activating Factor Receptor Transcript-1 but Not Transcript-2 by Human Bone Marrow Cells

Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France

Key Words. Bone marrow • CD34⁺ progenitors • Platelet-activating factor receptor • Stromal cells • RT-PCR • Bone marrow

Dr. Yves Denizot, Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr. Marcland, 87025 Limoges, France.

The presence of platelet-activating factor receptor (PAF-R) transcripts 1 and 2 was investigated in human bone marrow cells by a reverse transcriptase polymerase chain reaction (RT-PCR) procedure which detected their simultaneous presence. RT-PCR experiments reveal PAF-R transcript 1 (but not 2) in freshly isolated mononuclear marrow cells, CD34⁺ hematopoietic stem/progenitor cells and cultured marrow stromal cells. For these experiments, the 5637 human bladder carcinoma cell line is used as a positive control for the presence of PAF-R transcripts 1 and 2. Flow cytometry experiments confirm the presence of PAF-R on marrow stromal cells and CD34⁺ stem/progenitor cells. In conclusion, the expression of PAF-R transcript 1, which mainly exists in circulating leukocytes, is also found in CD34⁺ stem/progenitor cells and cells of the marrow microenvironment, strengthening the potential role of PAF during marrow hematopoiesis.

This article has been cited by other articles:

				M. Donnard, L. Guglielmi, P. Turlure, C. Piguet, M.J. Couraud, D. Bordessoule, and Y. Denizot Membrane and Intracellular Platelet-Activating Factor Receptor Expression in Leukemic Blasts of Patients with Acute Myeloid and Lymphoid Leukemia Stem Cells, September 1, 2002; 20(5): 394 - 401. [Abstract] [Full Text] [PDF]

				V. Desplat, J.-L. Faucher, F. X. Mahon, P. Dello Sbarba, V. Praloran, and Z. Ivanovic Hypoxia Modifies Proliferation and Differentiation of CD34+ CML Cells Stem Cells, July 1, 2002; 20(4): 347 - 354. [Abstract] [Full Text] [PDF]