HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dürig, J. Articles by Heyworth, C. M. Search for Related Content PubMed PubMed Citation Articles by Dürig, J. Articles by Heyworth, C. M.

Distinct Biological Effects of Macrophage Inflammatory Protein-1 and Stroma-Derived Factor-1 on CD34⁺ Hemopoietic Cells

^a Department of Haematology, University Hospital Essen, Essen, Germany;
^b CRC Section of Haemopoietic Cell and Gene Therapeutics, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom

Key Words. Chemokines • MIP-1 • SDF-1 • Hemopoietic cells • Calcium flux • Growth inhibition • Adhesion

Dr. Clare Heyworth, CRC Section of Haemopoietic Cell and Gene Therapeutics, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, United Kingdom.

Chemokines are important regulators of both hemopoietic progenitor cell (HPC) proliferation and adhesion to extracellular matrix molecules. Here, we compared the biological effects of the CC chemokine macrophage inflammatory protein-1 (MIP-1) with those of the CXC chemokine stroma-derived factor-1 (SDF-1) on immunomagnetically purified CD34⁺ cells from leukapheresis products (LP CD34⁺). In particular, studies on chemokine-induced alterations of LP CD34⁺ cell attachment to fibronectin-coated plastic surfaces, proliferation of these cells in colony-forming cell (CFC) assays and intracellular calcium mobilization were performed. MIP-1 but not SDF-1 was found to increase the adhesion of LP CD34⁺ cells to fibronectin in a dose-dependent manner. Both chemokines elicited growth-suppressive effects on LP CD34⁺ cells in CFC assays. While MIP-1 reduced the number of granulomonocytic (CFC-GM) and erythroid (BFU-E) colonies to the same extent, SDF-1 showed a significantly greater inhibitory effect on CFC-GM than BFU-E. Finally, we demonstrated that SDF-1 but not MIP-1 triggers increases in intracellular calcium in LP CD34⁺ cells. The SDF-1-induced calcium response was rapid and concentration-dependent, with a maximal stimulation observed at 15 ng/ml. In conclusion, our data suggest distinct biological properties of SDF-1 and MIP-1 in terms of modulation of LP CD34⁺ cell adhesion to fibronectin and intracellular calcium levels. However, comparable growth-suppressive effects on HPC proliferation were observed, indicating that this feature may be independent of chemokine-induced calcium responses.

This article has been cited by other articles:

				J.-J. Lataillade, D. Clay, C. Dupuy, S. Rigal, C. Jasmin, P. Bourin, and M.-C. L. Bousse-Kerdiles Chemokine SDF-1 enhances circulating CD34+ cell proliferation in synergy with cytokines: possible role in progenitor survival Blood, February 1, 2000; 95(3): 756 - 768. [Abstract] [Full Text] [PDF]