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Department of Immunology, Institute of Basic
Medical Sciences, Center for TARA, University of Tsukuba and CREST
(JST), Tsukuba Science-City, Ibaraki, Japan
* These two authors contributed equally to this work.
Key Words. Hematopoietic stem cells • NOD/SCID mouse • Cord blood • SCID-repopulating cell • CD34+ cells
Dr. Hiromitsu Nakauchi, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, Tsukuba Science-city, Ibaraki 305, Japan.
Although the hematopoietic activities of human CD34+ bone marrow (BM) and cord blood (CB) cells have been well characterized, the phenotype of nonobese-diabetic severe combined immunodeficient (NOD/SCID) mice repopulating cells (SRCs) in CB and BM has not yet been fully examined. To address this issue, various hematopoietic activities were compared in terms of total and CD34+ CB and BM cells. Clonal culture of fluorescence-activated cell sorter (FACS) CD34+ CB and BM cells revealed a higher incidence of colony-forming cells with greater proliferation capacity in CB over BM CD34+ cells. CB CD34+ cells also demonstrated higher secondary plating efficiency over BM cells. In addition, we demonstrated that mice transplanted with CB mononuclear cells (MNCs) showed significantly higher levels of chimerism than those transplanted with BM MNCs. However, recipients of FACS-sorted CD34+ CB cells showed significantly lower levels of chimerism than those that received total CB MNCs, suggesting a role of facilitating cells in the CD34– cell population. To further analyze the role of CD34– cells, the NOD/SCID repopulating ability of FACS-sorted CB CD34–c-kit+Lin– and CD34–c-kit-Lin– cells were examined. However, SRCs were not detected in those cells. Taken together, these data suggest that CB is a better source of hematopoietic stem cells and that there are cells in the CD34– fraction that facilitate repopulation of hematopoiesis in the NOD/SCID environment.
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