| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Osaka Red Cross Blood Center;
b Department of Pathology, Osaka Medical College;
c Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
Key Words. AC133 antigen • CD34+ cells • Peripheral blood • Proliferation • CFC • LTC-IC • Apoptosis
Kayoko Matsumoto, Ph.D., Osaka Red Cross Blood Center, Morinomiya 2-4-43, Johtoh-ku, Osaka, Japan 536-8505. Telephone: +81-6-6962-7056; Fax: +81-6-6962-7652; e-mail: kayakoma{at}a1.mbn.or.jp
AC133 antigen is a novel marker for human hematopoietic stem/progenitor cells. In this study, we examined the expression and proliferation potential of AC133+ cells obtained from steady-state peripheral blood (PB). The proportion of AC133+ cells in the CD34+ subpopulation of steady-state PB was significantly lower than that of cord blood (CB), although that of cytokine-mobilized PB was higher than that of CB. The proliferation potential of AC133+CD34+ and AC133–CD34+ cells was examined by colony-forming analysis and analysis of long-term culture-initiating cells (LTC-IC). Although the total number of colony-forming cells was essentially the same in the AC133+CD34+ fraction as in the AC133–CD34+ fraction, the proportion of LTC-IC was much higher in the AC133+CD34+ fraction. Virtually no LTC-IC were detected in the AC133–CD34+ fraction. In addition, the features of the colonies grown from these two fractions were quite different. Approximately 70% of the colonies derived from the AC133+CD34+ fraction were granulocyte-macrophage colonies, whereas more than 90% of the colonies derived from the AC133–CD34+ fraction were erythroid colonies. Furthermore, an ex vivo expansion study observed expansion of colony-forming cells only in the AC133+CD34+ population, and not in the AC133–CD34+ population. These findings suggest that to isolate primitive hematopoietic cells from steady-state PB, selection by AC133 expression is better than selection by CD34 expression.
This article has been cited by other articles:
|
|
 |
|
  K. Asosingh, S. Swaidani, M. Aronica, and S. C. Erzurum Th1- and Th2-Dependent Endothelial Progenitor Cell Recruitment and Angiogenic Switch in Asthma J. Immunol., May 15, 2007; 178(10): 6482 - 6494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Strathdee, A. Sim, R. Soutar, T. L. Holyoake, and R. Brown HOXA5 is targeted by cell-type-specific CpG island methylation in normal cells and during the development of acute myeloid leukaemia Carcinogenesis, February 1, 2007; 28(2): 299 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Okada, E. Matsuura, Z. Tozuka, R. Nagai, A. Watanabe, K. Matsumoto, K. Yasui, R. W. Jackman, T. Nakano, and T. Doi Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes Blood, October 1, 2004; 104(7): 2027 - 2034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Summers, C. M. Heyworth, E. A. de Wynter, C. A. Hart, J. Chang, and N. G. Testa AC133+ G0 Cells from Cord Blood Show a High Incidence of Long-Term Culture-Initiating Cells and a Capacity for More Than 100 Million-Fold Amplification of Colony-Forming Cells In Vitro Stem Cells, September 1, 2004; 22(5): 704 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Bohmer IL-3-Dependent Early Erythropoiesis Is Stimulated by Autocrine Transforming Growth Factor Beta Stem Cells, March 1, 2004; 22(2): 216 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yasui, K. Matsumoto, F. Hirayama, Y. Tani, and T. Nakano Differences Between Peripheral Blood and Cord Blood in the Kinetics of Lineage-Restricted Hematopoietic Cells: Implications for Delayed Platelet Recovery Following Cord Blood Transplantation Stem Cells, March 1, 2003; 21(2): 143 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Balducci, G. Azzarello, M. T. Valenti, G. M. Capuzzo, G. L. Pappagallo, I. Pilotti, S. Ausoni, M. Bari, F. Rosetti, D. Sartori, et al. The Impact of Progenitor Enrichment, Serum, and Cytokines on the Ex Vivo Expansion of Mobilized Peripheral Blood Stem Cells: A Controlled Trial Stem Cells, January 1, 2003; 21(1): 33 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Burger, S. Coetzee, W. L. McKeehan, M. Kan, P. Cook, Y. Fan, T. Suda, R. P. Hebbel, N. Novitzky, W. A. Muller, et al. Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells Blood, November 15, 2002; 100(10): 3527 - 3535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kominato, Y. Hata, H. Takizawa, K. Matsumoto, K. Yasui, J.-i. Tsukada, and F.-i. Yamamoto Alternative Promoter Identified between a Hypermethylated Upstream Region of Repetitive Elements and a CpG Island in Human ABO Histo-blood Group Genes J. Biol. Chem., September 27, 2002; 277(40): 37936 - 37948. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
