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Mobilization Kinetics of CD34⁺ Cells in Association with Modulation of CD44 and CD31 Expression during Continuous Intravenous Administration of G-CSF in Normal Donors

^a Departments of Hematology-Oncology,
^b Pediatrics and
^c Clinical Pathology, College of Medicine, Ewha Woman's University, Seoul, Korea;
^d Dept. of Cell Biology & Immunology, Asan Institute for Life Science, Seoul, Korea

Key Words. Continuous i.v. G-CSF • Mobilization kinetics • CD34⁺ cells • CD31 • CD44 • Normal donors

Chu-Myong Seong, M.D., Department of Hematology-Oncology, Ewha Woman's University Mock-Dong Hospital, Yangchun-Ku, Mock-6-Dong 911-1 Seoul, Korea 158-710. Telephone:82-2-650-5015; Fax: 82-2-650-5062; e-mail: pbpct{at}unitel.co.kr

The aim of the present study is to evaluate the kinetics of CD34⁺ cells and investigate the potential modulation of CD44 and CD31 expression on CD34⁺ cells during continuous i.v. administration of G-CSF, thus to elucidate the possible mechanism of peripheral blood progenitor cell (PBPC) mobilization. Fifteen healthy donors were enrolled in this study. G-CSF (10 µg/kg/day) was administered for four consecutive days through continuous 24-h i.v. infusion. For measurement of complete blood counts, CD34⁺ cell levels and their expression of CD44 and CD31, PB sampling was performed immediately before the administration of G-CSF (steady-state) and after 4, 8, 24, 48, 72, 96, and 120 h of G-CSF administration. The percentage and absolute number of CD34⁺ cells significantly increased at day 3 (0.55 ± 0.09%, 51.12 ± 24.83 x 10³/ml) and day 4 (0.47 ± 0.09%, 46.66 ± 24.93 x 10³/ml), compared to the steady-state level (0.06 ± 0.09%, 2.03 ± 5.69 x 10³/ml). At day 3 to day 5 following the onset of G-CSF administration, a strong decrease of CD44 and CD31 expression was observed on mobilized CD34⁺ cells compared to controls: the relative fluorescence intensity of CD44 and CD31 was, respectively, 50%-70% and 40%-90% lower than that of controls. We conclude that continuous i.v. administration of G-CSF apparently results in more rapid mobilization of CD34⁺ cells, and downregulation of CD44 and CD31 on CD34⁺ cells is likely to be involved in the mobilization of PBPC after treatment with G-CSF.