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Stem Cells, Vol. 19, No. 2, 144-150, March 2001
© 2001 AlphaMed Press

Successful Allogeneic Bone Marrow Transplantation (BMT) by Injection of Bone Marrow Cells via Portal Vein: Stromal Cells as BMT-Facilitating Cells

Tian-Xue Fana, Hiroko Hishaa,b, Tie-Nan Jina, Cheng-Ze Yua, Zhe-Xiong Liana, Shu-Bin Guoa, Yun-Ze Cuia, Biao Fenga, Guo-Xiang Yanga, Qing Lia, Susumu Ikeharaa,b

a First Department of Pathology,
b Transplantation Center, Kansai Medical University, Osaka, Japan

Key Words. Bone marrow transplantation • Hemopoietic stem cells • Bone marrow stromal cells • Portal vein

Susumu Ikehara, M.D., Ph.D., First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka 570-8506, Japan. Telephone: 81-6-6-6993-9429; Fax: 81-6-6994-8283; e-mail: ikehara{at}takii.kmu.ac.jp

We examined the importance of the coadministration of bone marrow (BM) stromal cells with BM cells via the portal vein. A significant increase in the number of day-14 colony-forming unit-spleen (CFU-S) was observed in the recipient mice injected with hemopoietic stem cells (HSCs) along with donor BM stromal cells obtained after three to four weeks of culture. Histological examination revealed that hematopoietic colonies composed of both donor hemopoietic cells and stromal cells coexist in the liver of these mice. However, when donor HSCs plus BM stromal cells were administered i.v., neither the stimulatory effects on CFU-S formation nor the hemopoietic colonies in the recipient liver were observed.

These findings suggest that the interaction of HSCs with stromal cells in the liver is the first crucial step for successful engraftment of allogeneic HSCs. It is likely that donor stromal cells and HSCs trapped in the liver migrate into the recipient BM and spleen, where they form CFU-BM and CFU-S, respectively.




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