HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Przyborski, S. A. Search for Related Content PubMed PubMed Citation Articles by Przyborski, S. A.

Isolation of Human Embryonal Carcinoma Stem Cells by Immunomagnetic Sorting

Department Biological Sciences, University of Durham, Durham, United Kingdom

Key Words. Pluripotent stem cell • Embryonal carcinoma • Isolation • Cloning • Neuron • Glia

Stefan Alexander Przyborski, Ph.D., Department Biological Sciences, University of Durham, South Road, Durham DH1 3LE, United Kingdom. Telephone: 44-0-191-3743341; Fax: 44-0-191-3742417; e-mail: stefan.przyborski{at}durham.ac.uk

Embryonal carcinoma cells are pluripotent stem cells derived from germ cell tumors and can be used to study cell differentiation in vitro. This report describes an approach designed to isolate pluripotent stem cells from primary/parent stock cultures of explanted tumor material. Cells expressing the pluripotent stem cell marker, SSEA-3, were isolated from heterogeneous stock cultures of the human teratoma line, TERA2, using immunomagnetic isolation. Single cell selection was performed on isolated SSEA-3⁺ cells and clonal lines were established. Each line was ultimately grown as a homogeneous monolayer, independent of feeder cells and expressed high levels of markers for pluripotent stem cells. In response to retinoic acid, clone TERA2.cl.SP-12 cells displayed enhanced neural differentiation compared to previously isolated TERA2 sublines and formed both neurons and glia. Deriving human pluripotent stem cell lines that differentiate into a range of cell types will provide useful tools to understand the molecular mechanisms controlling cell differentiation in a manner pertinent to human embryonic development.

This article has been cited by other articles:

				T.-W. D. Leong, F.-T. Chew, and D. W. Hutmacher Isolating Bone Marrow Stem Cells Using Sieve Technology Stem Cells, November 1, 2004; 22(6): 1123 - 1125. [Full Text] [PDF]

				S. Alexander Przyborski, S. Smith, and A. Wood Transcriptional Profiling of Neuronal Differentiation by Human Embryonal Carcinoma Stem Cells In Vitro Stem Cells, July 1, 2003; 21(4): 459 - 471. [Abstract] [Full Text] [PDF]

				R. Stewart, V. B. Christie, and S. A. Przyborski Manipulation of Human Pluripotent Embryonal Carcinoma Stem Cells and the Development of Neural Subtypes Stem Cells, May 1, 2003; 21(3): 248 - 256. [Abstract] [Full Text] [PDF]