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International Journal of Cell Cloning, Vol 2, 81-88, Copyright © 1984 by AlphaMed Press

ORIGINAL ARTICLES

Cytogenetic studies in dogs after total body irradiation and allogeneic transfusion with cryopreserved blood mononuclear cells: observations in long-term chimeras

F Carbonell, W Calvo, TM Fliedner, E Kratt, H Gerhartz, M Korbling, W Nothdurft and WM Ross

Cytogenetic studies were performed on two dog groups after total body irradiation and allogeneic transfusion with cryopreserved blood mononuclear cells. The first group of dogs was transfused with unseparated leukocytes and suffered from graft-versus-host disease (GvHD). Cytogenetic studies demonstrated only cells of donor origin in all dogs of this group. The second group of animals was transfused with fraction 2 of a discontinuous albumin gradient. The dogs of this group did not develop GvHD, and the cytogenetic studies showed the presence of a mosaic of cells from donor and recipient origin in all of them. These results suggest that the GvHD may suppress autochthonous regeneration.


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W. Bensinger, R. Clift, C Anasetti, F. Appelbaum, T Demirer, S Rowley, B. Sandmaier, B Torok-Storb, R Storb, and C. Buckner
Transplantation of allogeneic peripheral blood stem cells mobilized by recombinant human granulocyte colony stimulating factor
Stem Cells, January 1, 1996; 14(1): 90 - 105.
[Abstract]


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