International Journal of Cell Cloning, Vol 2, 185-197, Copyright © 1984 by AlphaMed Press
Effect of a neutrophilia-inducing tumor on hemopoietic stem cells in mice
MY Lee and NS Wolf
The influence of neutrophilic stimulation on hemopoietic stem cells was
studied in mice with tumor-induced neutrophilia. Transfusions of marrow
cells from normal and neutrophilic tumor-bearing mice into lethally
irradiated normal and tumor-bearing mice were performed. The number and the
erythroid:granuloid (E:G) ratio of day 7 colonies in the recipient spleens
and bones as well as the size of spleen colonies of recipient animals were
determined. The E:G ratio of spleen and bone marrow colonies between normal
and tumor-bearing mouse recipients and the number of spleen colonies did
not differ significantly in either experiment. However, spleen colonies
which developed in tumor-bearing irradiated mice were significantly larger
than those which developed in normal recipients in both experiments. These
studies indicated that while the line of differentiation taken by
hemopoietic stem cells was not affected by the neutrophilic influence of
the tumor, the tumor- bearing host environment appeared to enhance
proliferation of transfused stem cells and/or their descendants. The
stimulators of granulocytopoiesis in this model of neutrophilia appear to
act on a population of progenitor cells more mature than the stem cells
capable of forming 7-day colonies in the spleen and bone marrow of
irradiated recipient mice.
