International Journal of Cell Cloning, Vol 2, 254-262, Copyright © 1984 by AlphaMed Press
Chemotherapeutic susceptibility of human bone marrow progenitor cells and human myelogenous leukemia cells (HL-60) in co-culture: preliminary report
T Himori, T Ohnuma and JF Holland
We determined the chemotherapeutic susceptibility of normal human
granulocyte progenitor cells (CFU-C) and acute myelogenous leukemia cells
(HL-60) in co-culture. Nucleated bone marrow cells and HL-60 cells were
mixed in 0.3% agar containing McCoy's 5A medium, fetal bovine serum, human
placenta-conditioned medium, and various concentrations of chemotherapeutic
agents. They were incubated in 5% humidified CO2 at 37 degrees C for 8-10
days. CFU-C and HL-60 colonies were differentiated morphologically. The
formation of CFU-C was progressively inhibited with the increasing number
of HL-60 cells, whereas the presence or absence of bone marrow cells did
not influence the number of HL-60 colonies. In separate culture, HL-60
cells were more sensitive to vincristine than were CFU-C cells. In
co-culture, however, the CFU-C became more sensitive to vincristine than in
the separate culture. Similarly, co-culture CFU-C were more sensitive to
daunorubicin than in separate culture. These data indicate that HL-60
leukemic cells exert inhibitory effects on normal bone marrow CFU-C; in
such an inhibited state, normal bone marrow is more susceptible to certain
chemotherapeutic agents.
