International Journal of Cell Cloning, Vol 2, 263-271, Copyright © 1984 by AlphaMed Press

ORIGINAL ARTICLES

Experimental immunologically mediated aplastic anemia (AA) in mice: cyclosporin A fails to protect against AA

WH Knospe, D Steinberg, A Gratwohl and B Speck

Immunologically mediated aplastic anemia (AA) in mice was induced by the i.v. injection of 10(7) lymph node cells (LNC) from H-2k identical but Mls mismatched CBA/J donor mice into previously irradiated (600 rad total body gamma) C3H/HeJ mice. Cyclosporin A (CsA), 25 mg/kg, was administered subcutaneously from day -1 to day 30. Control mice included C3H/HeJ mice which received 600 rad alone, C3H/HeJ mice which received 600 rad plus CsA as above, and C3H/HeJ mice which received 600 rad total body irradiation followed by 10(7) LNC from CBA/J donors. CsA failed to prevent lethal AA. These results suggest that the pathogenetic mechanisms operating in immunologically mediated AA differ from the mechanisms operating in rodents transplanted with allogeneically mismatched marrow or spleen cells which develop graft- versus-host disease. The results are consistent with a non-T cell- dependent mechanism causing the AA.

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