| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Departments of Anatomy and Cell Biology and
b Dermatology, The University of Iowa, Iowa City, Iowa, USA
Key Words. Stem cells • Epidermis • Pluripotent • Plasticity • Development
Jackie R. Bickenbach, Ph.D., Departments of Anatomy and Cell Biology, and Dermatology, The University of Iowa College of Medicine, 1-457 BSB, 51 Newton Road, Iowa City, IA 52242, USA. Telephone: 319-335-6719; Fax: 319-335-7198; e-mail: jackie-bickenbach{at}uiowa.edu
It has been demonstrated that several types of somatic stem cells have the remarkable capacity to differentiate into other types of tissues. We demonstrate here that stem cells from the skin, the largest organ of the body, have the capacity to form multiple cell lineages during development. Using our recently developed sorting technique, we isolated viable homogenous populations of somatic epidermal stem and transient amplifying cells from the skin of 3-day old transgenic mice, who carried the enhanced green fluorescent protein transgene, and injected stem, TA, or unsorted basal epidermal cells into 3.5-day C57BL/6 blastocysts. Only the stem-injected blastocysts produced mice with GFP+ cells in their tissues. We found GFP+ cells in ectodermal, mesenchymal, and neural-crest-derived tissues in E13.5 embryos, 13-day-old neonates, and 60-day-old adult mice, proving that epidermal stem cells survived the blastocyst injection and multiplied during development. Furthermore, the injected stem cells altered their epidermal phenotype and expressed the appropriate proteins for the tissues into which they developed, demonstrating that somatic epidermal stem cells have the ability to produce cells of different lineages during development. These data suggest that somatic epidermal stem cells may show a generalized plasticity expected only of embryonic stem cells and that environmental (extrinsic) factors may influence the lineage pathway for somatic stem cells. Thus, the skin could be a source of easily accessible stem cells that are able to be reprogrammed to form multiple cell lineages.
This article has been cited by other articles:
|
|
 |
|
  M. Miyazaki, M. Hardjo, T. Masaka, K. Tomiyama, N. Mahmut, R. J. Medina, A. Niida, H. Sonegawa, G. Du, R. Yong, et al. Isolation of a Bone Marrow-Derived Stem Cell Line with High Proliferation Potential and Its Application for Preventing Acute Fatal Liver Failure Stem Cells, November 1, 2007; 25(11): 2855 - 2863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wan, M. Yuan, C. Simpson, K. Allen, F. N.E. Gavins, M. S. Ikram, S. Basu, N. Baksh, E. A. O'Toole, and I. R. Hart Stem/Progenitor Cell-Like Properties of Desmoglein 3dim Cells in Primary and Immortalized Keratinocyte Lines Stem Cells, May 1, 2007; 25(5): 1286 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-E. Zaragosi, G. Ailhaud, and C. Dani Autocrine Fibroblast Growth Factor 2 Signaling Is Critical for Self-Renewal of Human Multipotent Adipose-Derived Stem Cells Stem Cells, November 1, 2006; 24(11): 2412 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-x. Zhou, L.-w. Jia, W.-m. Liu, C.-l. Miao, S. Liu, Y.-j. Cao, and E.-k. Duan Role of sonic hedgehog in maintaining a pool of proliferating stem cells in the human fetal epidermis Hum. Reprod., July 1, 2006; 21(7): 1698 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Illa-Bochaca and L. M. Montuenga The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells J. Exp. Biol., June 1, 2006; 209(11): 2215 - 2223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yu, D. Fang, S. M. Kumar, L. Li, T. K. Nguyen, G. Acs, M. Herlyn, and X. Xu Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles Am. J. Pathol., June 1, 2006; 168(6): 1879 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. E. Sigurjonsson, M.-C. Perreault, T. Egeland, and J. C. Glover Adult human hematopoietic stem cells produce neurons efficiently in the regenerating chicken embryo spinal cord PNAS, April 5, 2005; 102(14): 5227 - 5232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Greco, H. P. Low, E. C. Johnson, R. A. Salmonsen, J. Gallant, S. N. Jones, A. H. Ross, and L. D. Recht Differentiation Prevents Assessment of Neural Stem Cell Pluripotency after Blastocyst Injection Stem Cells, July 1, 2004; 22(4): 600 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Prindull and D. Zipori Environmental guidance of normal and tumor cell plasticity: epithelial mesenchymal transitions as a paradigm Blood, April 15, 2004; 103(8): 2892 - 2899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-x. Zhou, S.-y. Chen, W.-m. Liu, Y.-j. Cao, and E.-k. Duan Enrichment and identification of human 'fetal' epidermal stem cells Hum. Reprod., April 1, 2004; 19(4): 968 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Jiao, S. Bronner, K. L. N. Mercer, D. D. Sheriff, G. C. Schatteman, and M. Dunnwald Epidermal cells accelerate the restoration of the blood flow in diabetic ischemic limbs J. Cell Sci., March 1, 2004; 117(7): 1055 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Montanaro, K. Liadaki, J. Volinski, A. Flint, and L. M. Kunkel Skeletal muscle engraftment potential of adult mouse skin side population cells PNAS, August 5, 2003; 100(16): 9336 - 9341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Rosenthal Prometheus's Vulture and the Stem-Cell Promise N. Engl. J. Med., July 17, 2003; 349(3): 267 - 274. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
