Stem Cells
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First published online September 15, 2005
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Submitted on April 1, 2005
Accepted on August 28, 2005

Rapid Communication

Analysis of Esg1 expression in pluripotent cells and the germline reveals similarities with Oct4 and Sox2 and differences between human pluripotent cell lines

Patrick S. Western 1*, Joanna Maldonado-Saldivia 2, Jocelyn van den Bergen 1, Petra Hajkova 2, Mitinori Saitou 3, Sheila C. Barton 2, M. Azim Surani 2

1 Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Victoria, Australia, 3052.
2 Wellcome Trust Cancer Research UK Gurdon Institute, and Department of Physiology, Tennis Court Road, University of Cambridge, Cambridge CB2 1QR, UK.
3 Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan

* To whom correspondence should be addressed. E-mail: patrick.western{at}mcri.edu.au.


  Abstract

Establishment of pluripotent epiblast cells is a critical event during early mammalian development, since all somatic lineages and the primordial germ cells (PGCs) are derived from them. The epiblast and PGCs are in turn the precursors of pluripotent embryonic stem cells (ES) and embryonic germ cells (EG), respectively. Although PGCs are specialised cells, they express of a number of key pluripotency-related genes such as Oct4 and Sox2. We have analysed Esg1 expression in mouse and human cells and shown that in the mouse the gene is specifically expressed in pre-implantation embryos, stem cells and the germline. Moreover, Esg1 co-expresses with Oct4 and Sox2, confirming its identity as a marker of the pluripotent cycle. Esg1 is also expressed with Oct4 and Sox2 by hES cells and germ cell carcinoma tissue but not all hEC cell lines. This data suggests that together with Oct4 and Sox2, Esg1 plays a conserved role in the pluripotent pathway of mouse and human stem and germ cells.




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