| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Tissue-Specific Stem Cells |
1 Centre for Women's Health Research, Monash Institute of Medical Research and Monash University Department of Obstetrics and Gynecology, Monash Medical Centre, Clayton Victoria, Australia
* To whom correspondence should be addressed. E-mail: caroline.gargett{at}med.monash.edu.au.
 |
Abstract |
|---|
Human and mouse endometrium (lining of the uterus) undergo cycles of growth and regression as part of each reproductive cycle. A well-known method to identify somatic stem/progenitor cells and their location in the stem cell niche is the label retaining cell approach (LRC). We hypothesized that mouse endometrium contains small populations of both epithelial and stromal somatic stem/progenitor cells which may be detected by the LRC technique. The overall objective of this study was to identify and quantify LRCs in mouse endometrium, to determine their location and identify their niche in this highly regenerative tissue. Endometrium was labeled for 3 days with bromodeoxyuridine (BrdU) in postnatal (P3) prior to gland development and prepubertal (P19) mice after glands had formed, followed by chase periods of up to 12 weeks. After an 8-week chase, 3% of epithelial nuclei immunostained with BrdU antibody and were considered epithelial LRCs. These were primarily located in the luminal epithelium. Epithelial LRCs did not express estrogen receptor-
(ER-). Stromal LRCs (6%) were found adjacent to luminal epithelium, at the endometrial-myometrial junction and near blood vessels after a 12-week chase. Stromal LRCs were Sca-1-, CD45-, and some (16%) expressed ER-, indicating their capacity to respond to estrogen and transmit paracrine signals to epithelial cells for endometrial epithelium regeneration. Both epithelial LRC and some stromal LRCs mainly located at the endometrial-myometrial junction were recruited into cell cycle after estrogen-stimulated endometrial regeneration, indicating a functional response to proliferative signals. This study has demonstrated for the first time the presence of both epithelial and stromal LRCs in mouse endometrium suggesting that these stemlike cells may be responsible for endometrial regeneration.
This article has been cited by other articles:
|
|
 |
|
  S. Muffler, H.-J. Stark, M. Amoros, B. Falkowska-Hansen, K. Boehnke, H.-J. Buhring, A. Marme, J. R. Bickenbach, and P. Boukamp A Stable Niche Supports Long-Term Maintenance of Human Epidermal Stem Cells in Organotypic Cultures Stem Cells, October 1, 2008; 26(10): 2506 - 2515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. da Silva Meirelles, A. I. Caplan, and N. B. Nardi In Search of the In Vivo Identity of Mesenchymal Stem Cells Stem Cells, September 1, 2008; 26(9): 2287 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Kurzrock, D. K. Lieu, L. A. deGraffenried, C. W. Chan, and R. R. Isseroff Label-retaining cells of the bladder: candidate urothelial stem cells Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1415 - F1421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Dimitrov, T Timeva, D Kyurkchiev, M Stamenova, A Shterev, P Kostova, V Zlatkov, I Kehayov, and S Kyurkchiev Characterization of clonogenic stromal cells isolated from human endometrium Reproduction, April 1, 2008; 135(4): 551 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ono, T. Maruyama, H. Masuda, T. Kajitani, T. Nagashima, T. Arase, M. Ito, K. Ohta, H. Uchida, H. Asada, et al. Side population in human uterine myometrium displays phenotypic and functional characteristics of myometrial stem cells PNAS, November 20, 2007; 104(47): 18700 - 18705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.E. Schwab and C.E. Gargett Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium Hum. Reprod., November 1, 2007; 22(11): 2903 - 2911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bratincsak, M. J. Brownstein, R. Cassiani-Ingoni, S. Pastorino, I. Szalayova, Z. E. Toth, S. Key, K. Nemeth, J. Pickel, and E. Mezey CD45-Positive Blood Cells Give Rise to Uterine Epithelial Cells in Mice Stem Cells, November 1, 2007; 25(11): 2820 - 2826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Kaitu'u-Lino, N. B. Morison, and L. A. Salamonsen Estrogen Is Not Essential for Full Endometrial Restoration after Breakdown: Lessons from a Mouse Model Endocrinology, October 1, 2007; 148(10): 5105 - 5111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. F. Wolff, A. B. Wolff, Hongling Du, and H. S. Taylor Demonstration of Multipotent Stem Cells in the Adult Human Endometrium by In Vitro Chondrogenesis Reproductive Sciences, September 1, 2007; 14(6): 524 - 533. [Abstract] [PDF]
|
|
|
|
|
|
C. E. Gargett Review Article: Stem Cells in Human Reproduction Reproductive Sciences, July 1, 2007; 14(5): 405 - 424. [Abstract] [PDF]
|
|
|
|
|
|
P. P. Szotek, H. L. Chang, L. Zhang, F. Preffer, D. Dombkowski, P. K. Donahoe, and J. Teixeira Adult Mouse Myometrial Label-Retaining Cells Divide in Response to Gonadotropin Stimulation Stem Cells, May 1, 2007; 25(5): 1317 - 1325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Masuda, T. Maruyama, E. Hiratsu, J. Yamane, A. Iwanami, T. Nagashima, M. Ono, H. Miyoshi, H. J. Okano, M. Ito, et al. Noninvasive and real-time assessment of reconstructed functional human endometrium in NOD/SCID/{gamma}Formula immunodeficient mice PNAS, February 6, 2007; 104(6): 1925 - 1930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Cervello, J.A. Martinez-Conejero, J.A. Horcajadas, A. Pellicer, and C. Simon Identification, characterization and co-localization of label-retaining cell population in mouse endometrium with typical undifferentiated markers Hum. Reprod., January 1, 2007; 22(1): 45 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.E. Gargett Uterine stem cells: What is the evidence? Hum. Reprod. Update, January 1, 2007; 13(1): 87 - 101. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
