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Embryonic Stem Cells |
1 Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
* To whom correspondence should be addressed. E-mail: christer.hoog{at}ki.se.
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Abstract |
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Several recent studies have suggested that mouse embryonic stem cells (ESCs) can differentiate into female and male germ cells in vitro. The meiotic process in germ cell-like cells derived from ESCs has not been studied in detail, but it has been reported that SYCP3 is expressed in these cells. Here we have carefully evaluated the meiotic process in germ cell-like cells derived from ESCs using a panel of meiosisspecific markers that identify distinct meiotic signatures unique to meiotic prophase I development in vivo. We find that whereas SYCP3 is expressed in germ cell-like cells, other meiotic proteins such as SYCP1, SYCP2, STAG3, REC8 and SMC1
are not expressed. The nuclear distribution of SYCP3 in the germ cell-like cell is highly
abnormal and not associated with the chromosomes of these cells. Fluorescence In Situ Hybridization (FISH) analysis shows that the SYCP3-positive germ cell-like cells do not contain synapsed homologous chromosomes, but instead display a chromosomal organization normally found in somatic cells. The absence of expression of essential meiotic proteins and a normal meiotic chromosomal organization strongly suggest that the germ cell-like cells formed from ESCs fail to progress through meiosis.
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