Stem Cells
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First published online October 19, 2006
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2005-0556v1
25/2/340    most recent
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Submitted on November 10, 2005
Accepted on October 10, 2006

Tissue-Specific Stem Cells

NG2 and olig2 expression provides evidence for phenotypic deregulation of cultured CNS and PNS neural precursor cells

Cecile Dromard 1, Sylvain Bartolami 1, loic Deleyrolle 1, Hirohide Takebayashi 2, Chantal Ripoll 1, Lionel Simonneau 1, Sylvie Prome 1, Sylvie Puech 1, Christophe Tran Van Ba 1, Christophe Duperray 3, Jean Valmier 1, Alain Privat 1, Jean Philippe Hugnot 1*

1 INSERM U583, Physiopathologie et Thérapie des déficits sensoriels et moteurs, Institut des Neurosciences de Montpellier, Hôpital St ELOI, Montpellier, France
2 Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
3 Service Regional INSERM de cytométrie en flux, Montpellier, France

* To whom correspondence should be addressed. E-mail: hugnot{at}univ-montp2.fr.


  Abstract

Neural stem cells cultured with FGF2/EGF generate clonal expansions called neurospheres (NS) which are widely used for therapy in animal models. However, their cellular composition is still poorly defined. Here, we report that NS derived from several embryonic and adult central nervous system (CNS) regions are mainly composed of remarkable cells coexpressing radial glia markers (BLBP, RC2, GLAST), oligodendrogenic/neurogenic factors (Mash1, Olig2, Nkx2.2) and markers which in vivo are typical of the oligodendrocyte lineage (NG2, A2B5, PDGFR{alpha}). On NS differentiation, the latter remain mostly expressed in neurons, together with Olig2 and Mash1. Using cytometry, we show that in growing NS the small population of multipotential self-renewing NS-forming cells are A2B5+ and NG2+. Additionally, we demonstrate that these NS-forming cells in the embryonic spinal cord were initially NG2- and rapidly acquired NG2 in vitro. NG2 and Olig2 were found to be rapidly induced by cell culture conditions in spinal cord neural precursor cells. Olig2 expression was also induced in astrocytes and embryonic peripheral nervous system (PNS) cells in culture following EGF/FGF treatment. These data provide new evidence for profound phenotypic modifications in CNS and PNS neural precursor cells induced by culture conditions.

Key Words. Neural stem cells, oligodendrocytes, NG2, proteoglycan, peripheral nervous system, neurospheres, cytometry, plasticity




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[Abstract] [Full Text] [PDF]


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