Stem Cells
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online March 23, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2005-0629v1
2005-0629v2
24/7/1822    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gordon, M.Y.
Right arrow Articles by Habib, N.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gordon, M.Y.
Right arrow Articles by Habib, N.A.
Submitted on December 16, 2005
Accepted on March 8, 2006

Translational and Clinical Research

CHARACTERISATION AND CLINICAL APPLICATION OF HUMAN CD34+ STEM/PROGENITOR CELL POPULATIONS MOBILISED INTO THE BLOOD BY G-CSF

M.Y. Gordon 1, N. Levicar 2, M. Pai 2, P. Bachellier 3, I. Dimarakis 2, F. Al-Allaf 2, H. M'Hamdi 2, T. Thalji 2, J.P. Welsh 2, S.B. Marley 2, J. Davis 2, F. Dazzi 2, F. Marelli-Berg 2, P. Tait 2, R. Playford 2, L. Jiao 2, S. Jensen 2, J.P. Nicholls 1, A. Ayav 2, M. Nohandani 2, F. Farzaneh 4, J. Gaken 4, R. Dodge 5, M. Alison 6, J.F. Apperley 2, R. Lechler 4, N.A. Habib 1*

1 Faculty of Medicine, Imperial College London, Hammersmith Campus, London, United Kingdom; LiverCyte Limited, London, United Kingdom
2 Faculty of Medicine, Imperial College London, Hammersmith Campus, London, United Kingdom
3 LiverCyte Limited, London, United Kingdom
4 Faculty of Medicine, Kings, Guy's and St Thomas's Medical School, King's College Campus, London, United Kingdom
5 Harvard Medical School, Boston, Massecheusetts; Joslin Diabetes Centre, Las Vegas, Nevada
6 The Royal London Hospital, Diabetes and Metabolic Medicine, London, United Kingdom

* To whom correspondence should be addressed. E-mail: nagy.habib{at}imperial.ac.uk.


  Abstract

A phase I study was performed to determine the safety and tolerability of injecting autologous CD34+ cells into five patients with liver insufficiency. The study was based on the hypothesis that the CD34+ cell population in G-CSF mobilised blood contains a subpopulation of cells with the potential for regenerating damaged tissue. We separated a candidate CD34+ stem cell population from the majority of the CD34+ cells (99%) by adherence to tissue culture plastic. The adherent and non-adherent CD34+ cells were distinct in morphology, immunophenotype and gene expression profile. RT-PCR-based gene expression analysis indicated that the adherent CD34+ cells had the potential to express determinants consistent with liver, pancreas, heart, muscle and nerve cell differentiation as well as hematopoiesis. Overall, the characteristics of the adherent CD34+ cells identify them as a separate putative stem/progenitor cell population. In culture, they produced a population of cells exhibiting diverse morphologies and expressing genes corresponding to multiple tissue types. Encouraged by this evidence that the CD34+ cell population contains cells with the potential to form hepatocyte-like cells, five patients with liver insufficiency were given G-CSF to mobilise their stem cells for collection by leukapheresis. Between 1 x 106 and 2 x 108 CD34+ cells were injected into the portal vein (3 patients) or hepatic artery (2 patients). No complications or specific side effects related to the procedure were observed. Three of the five patients showed improvement in serum bilirubin and four of five in serum albumin. These observations warrant further clinical trials.

Key Words. Stem cells, liver disease, transplantation, regenerative medicine




This article has been cited by other articles:


Home page
 Hum ReprodHome page
M. A. Mikhail, H. M'Hamdi, J. Welsh, N. Levicar, S. B. Marley, J. P. Nicholls, N. A. Habib, L. S. Louis, N. M. Fisk, and M. Y. Gordon
High frequency of fetal cells within a primitive stem cell population in maternal blood
Hum. Reprod., April 1, 2008; 23(4): 928 - 933.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Bonig, A. Wundes, K.-H. Chang, S. Lucas, and T. Papayannopoulou
Increased numbers of circulating hematopoietic stem/progenitor cells are chronically maintained in patients treated with the CD49d blocking antibody natalizumab
Blood, April 1, 2008; 111(7): 3439 - 3441.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
S. J Forbes
Stem cell therapy for chronic liver disease choosing the right tools for the job
Gut, February 1, 2008; 57(2): 153 - 155.
[Full Text] [PDF]

Home page
 GutHome page
A. C. Lyra, M. B. P. Soares, R. R. dos Santos, and L. G. C. Lyra
Bone marrow stem cells and liver disease
Gut, November 1, 2007; 56(11): 1640 - 1640.
[Full Text] [PDF]

Home page
 Stem CellsHome page
S. Lorenzini and P. Andreone
Stem Cell Therapy for Human Liver Cirrhosis: A Cautious Analysis of the Results
Stem Cells, September 1, 2007; 25(9): 2383 - 2384.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
Y N Kallis, M R Alison, and S J Forbes
Bone marrow stem cells and liver disease
Gut, May 1, 2007; 56(5): 716 - 724.
[Full Text] [PDF]

Home page
 Stem CellsHome page
R. M. Lemoli, L. Catani, S. Talarico, E. Loggi, A. Gramenzi, U. Baccarani, M. Fogli, G. L. Grazi, M. Aluigi, G. Marzocchi, et al.
Mobilization of Bone Marrow-Derived Hematopoietic and Endothelial Stem Cells After Orthotopic Liver Transplantation and Liver Resection
Stem Cells, December 1, 2006; 24(12): 2817 - 2825.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS

Copyright © 2006 by AlphaMed Press.