Stem Cells http://www.peprotech.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online December 21, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2006-0615v1
25/4/986    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wakayama, S.
Right arrow Articles by Wakayama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wakayama, S.
Right arrow Articles by Wakayama, T.
Submitted on September 29, 2006
Accepted on December 12, 2006

Embryonic Stem Cells

Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies

Sayaka Wakayama 1, Takafusa Hikichi 1, Rinako Suetsugu 1, Yuko Sakaide 1, Hong-Thuy Bui 1, Eiji Mizutani 1, Teruhiko Wakayama 1*

1 Center for Developmental Biology, RIKEN Kobe, Kobe, Japan

* To whom correspondence should be addressed. E-mail: teru{at}cdb.riken.jp.


  Abstract

Recently, ES cell lines were established from single blastomeres taken from eight-cell embryos in mice and humans with success rates of 4% and 2%, respectively, which suggests that the method could be used in regenerative medicine to reduce ethical concerns over harm to embryos. However, those studies used other ES cells as supporting cells. Here, we report a simple and highly efficient method of establishing mouse ES cell lines from single blastomeres, in which single blastomeres are simply plated onto a feeder layer of mouse embryonic fibroblasts with modified ES cell medium. A total of 112 ES cell lines were established from two-cell (establishment rate, 50%-69%), early four-cell (28%-40%), late four-cell (22%), and eight-cell (14%-16%) stage embryos. We also successfully established 18 parthenogenetic ES cell lines from first (36%-40%) and second polar bodies (33%), the nuclei of which were reconstructed to embryos by nuclear transfer. Most cell lines examined maintained normal karyotypes and expressed markers of pluripotency, including germline transmission in chimeric mice. Our results suggest that the single cells of all early-stage embryos or polar bodies have the potential to be converted into ES cells without any special treatment.

Key Words. ES cell, blastomere, polar body, nuclear transfer




This article has been cited by other articles:


Home page
 Genes Dev.Home page
J. Yu and J. A. Thomson
Pluripotent stem cell lines
Genes & Dev., August 1, 2008; 22(15): 1987 - 1997.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
C. Lorthongpanich, S.-H. Yang, K. Piotrowska-Nitsche, R. Parnpai, and A. W S Chan
Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells
Reproduction, June 1, 2008; 135(6): 805 - 813.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. J. French, C. A. Adams, L. S. Anderson, J. R. Kitchen, M. R. Hughes, and S. H. Wood
Development of Human Cloned Blastocysts Following Somatic Cell Nuclear Transfer with Adult Fibroblasts
Stem Cells, February 1, 2008; 26(2): 485 - 493.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
Email Content Delivery
Copyright © 2006 by AlphaMed Press.