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CANCER STEM CELLS |
1 Dept. of Pediatrics and Pediatric Stem Cell Research Institute, Sheba Medical Center, Tel Hashomer, Israel
2 Dept. of Pediatrics and Pediatric Stem Cell Research Institute, Sheba Medical Center, Tel Hashomer, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
3 Department of Nephrology and Hypertension, Medical Faculty of the Charité and HELIOS Klinikum Berlin, Max-Delbrueck-Center for Molecular Medicine, Berlin-Buch, Germany
4 Dept. of Pediatric Hemato-Oncology and Sheba Cancer Research Center, Sheba Medical Center, Tel Hashomer, Israel
5 Dept. of Pediatric Hemato-Oncology and Sheba Cancer Research Center, Sheba Medical Center, Tel Hashomer, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
* To whom correspondence should be addressed. E-mail: benjamin.dekel{at}gmail.com.
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Abstract |
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Recent studies indicate a dual epigenetic role of the Polycomb group (PcG) proteins in self-renewal of stem cells and oncogenesis. Their elevation in our previous human kidney microarray screen led us examine whether they participate in processes involving normal and malignant renal progenitors. We therefore analyzed the expression of the PcG genes (EZH2, BMI-1, EED, SUZ12) in relation with that of the nephric-progenitor genes (WT1, PAX2, SALL1, SIX2, CITED1) using real-time PCR and methylation assays during renal development, regeneration and tumorigenesis.
While all of the nephric-progenitor genes were shown to be developmentally regulated, analysis of polycomb gene expression during murine nephrogenesis and in an in vitro induction model of the nephrogenic mesenchyme, indicated dynamic regulation only for EZH2 in the normal renal progenitor population. In contrast, induction of adult kidney regeneration by ischemia/reperfusion injury resulted primarily in rapid elevation of BMI-1 whereas EZH2 was silenced. Analysis of renal tumorigenesis in stem-cell like tumor xenografts established by serial passage of Wilms' tumor (WT) in immunodeficient mice, showed cooperative upregulation of all PcG genes. This was accompanied by up-regulation of WT1, PAX2, SALL1 but downregulation of SIX2. Accordingly, methylation-specific qPCR demonstrated promoter hypomethylation of WT1, PAX2 and SIX2 in primary WT and fetal kidneys, while progressive WT xenografts showed hypermethylation of SIX2, possibly leading to loss of renal differentiation.
PcG genes vary in expression during renal development, regeneration and tumorigenesis. We suggest a link between polycomb activation and epigenetic alterations of the renal progenitor population in initiation and progression of renal cancer.
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Author contributions: S.M.: Collection and/or assembly of data, Data analysis and interpretation; N.P.: Collection and/or assembly of data, Data analysis and interpretation; K.M.S.: Collection and/or assembly of data, Data analysis and interpretation; G.K.: Technologic advising; G.R.: financial support, consulting and advising; D.B.: Collection and/or assembly of data; B.D.: Conception and design, financial support, Data analysis and interpretation, manuscript writing.
Key Words. Polycomb, Stem cells, Wilms tumor, renal stem cells, xenograft
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