Stem Cells
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online November 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
2007-0406v1
2007-0406v2
26/2/550    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, C. P.
Right arrow Articles by Yang, Y. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, C. P.
Right arrow Articles by Yang, Y. C.
Submitted on May 24, 2007
Accepted on October 23, 2007

TRANSLATIONAL AND CLINICAL RESEARCH

Trafficking of Multipotent Mesenchymal Stromal Cells From Maternal Circulation Through the Placenta Involves VEGFR-1 and Integrins

Chie Pein Chen 1, Ming Yi Lee 2, Jian Pei Huang 3, John D. Aplin 4, Yi Hsin Wu 2, Cing Siang Hu 2, Pei Chun Chen 2*, Hung Li 5, Shiaw Min Hwang 6, Shu Hsiang Liu 2, Yuh Cheng Yang 2

1 Division of High Risk Pregnancy and Department of Medical Research, Mackay Memorial Hospital, Taipei 104, Taiwan. Mackay Medicine, Nursing and Management College, Taipei 112, Taiwan
2 Department of Medical Research, Mackay Memorial Hospital, Taipei 104, Taiwan
3 Division of High Risk Pregnancy, Mackay Memorial Hospital, Taipei 104, Taiwan
4 Division of Human Development, Medical School, University of Manchester, Manchester M13 0JH, United Kingdom
5 Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
6 Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan

* To whom correspondence should be addressed. E-mail: cpchen{at}ms2.mmh.org.tw.


  Abstract

Maternal cells can become engrafted in various fetal organs during pregnancy. The nature of the cells and the mechanisms of maternofetal cell trafficking are not clear. We demonstrate that human lineage-negative, CD34-negative (Lin-CD34-) multipotent mesenchymal stromal cells express {alpha}2, {alpha}4, {alpha}5, and {beta}1 integrins, which mediate their adhesion to endothelium, and vascular endothelial cell growth factor receptor-1 (VEGFR-1), which mediates their response to vascular endothelial cell growth factor A (VEGF-A). A maternal-fetal VEGF-A concentration gradient exists across the placental barrier, and cord blood plasma induces transendothelial and trans-Matrigel migration of stem cells in vitro. Migration is inhibited by a VEGF-A-neutralizing antibody or antibodies against VEGFR-1, integrin {alpha}2, {alpha}4, {alpha}5 or {beta}1. When Lin-CD34- multipotent mesenchymal stromal cells are transferred to rat maternal venous blood, they traffic through the placenta, engraft in various fetal organs and persist in offspring for at least 12 weeks. Cell proliferation ability is retained in the xenogeneic placenta. Maternofetal trafficking is significantly reduced by blocking antibodies against integrins {alpha}2, {alpha}4, {alpha}5, and {beta}1, or VEGFR-1. These results suggest that maternal microchimerism arises by the trafficking of multipotent mesenchymal stromal cells via VEGF-A- and integrin-dependent pathways across the hemochorial placenta to fetal tissues.

Key Words. integrins, multipotent mesenchymal stromal cell, microchimerism, placenta, vascular endothelial cell, growth factor receptor




This article has been cited by other articles:


Home page
 Hum ReprodHome page
C.-P. Chen, S.-H. Liu, J.-P. Huang, J. D. Aplin, Y.-H. Wu, P.-C. Chen, C.-S. Hu, C.-C. Ko, M.-Y. Lee, and C.-Y. Chen
Engraftment potential of human placenta-derived mesenchymal stem cells after in utero transplantation in rats
Hum. Reprod., January 1, 2009; 24(1): 154 - 165.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 2007 by AlphaMed Press.