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Liver-Specific Gene Expression in Cultured Human Hematopoietic Stem Cells

^a Bone Marrow Transplantation Center and
^b Department of Pediatric Surgery, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany

Key Words. Hepatic stem cells • Adult bone marrow stem cells • Stem cell culture

Henning Fiegel, M.D., Department of Pediatric Surgery, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Telephone: 49-40-42803-3494/6918; Fax: 49-40-42803-6914; e-mail: fiegel{at}uke.uni-hamburg.de

Hematopoietic and hepatic stem cells share characteristic markers such as CD34, c-kit, and Thy1. Based on the recent observations that hepatocytes may originate from bone marrow, we investigated the potential of CD34⁺ bone marrow cells to differentiate into hepatocytic cells in vitro. CD34⁺ and CD34^- human bone marrow cells were separated by magnetic cell sorting. Cells were cultured on a collagen matrix in a defined medium containing hepatocyte growth factor. Cell count and size were measured by flow cytometry, and reverse transcription polymerase chain reaction was carried out for the liver-specific markers CK-19 and albumin. During cell culture, CD34⁺ cells showed an increasing cell number and proliferative activity as assessed by Ki-67 staining. Under the specified culture conditions, CD34⁺ cells expressed albumin RNA and CK-19 RNA after 28 days, whereas CD34^- cells did not show liver-specific gene expression. The results indicate that CD34⁺ adult human bone marrow stem cells can differentiate into hepatocytic cells in vitro.

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