| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Departments of Parasitology and
b Neurosurgery, Nara Medical University, Nara, Japan
Key Words. Parkinson’s disease • ES cells • Transplantation • Apomorphine-induced rotational behavior • Tyrosine hydroxylase
Correspondence:
Masahide Yoshikawa, M.D., Division of Developmental Biology, Department of Parasitology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. Telephone: 81-744-22-3051 x2250; Fax: 81-744-24-7122; e-mail: myoshika{at}nmu-gw.naramed-u.ac.jp
Background and Aims. The purpose of the present study was to examine the efficacy of transplantation of mouse embryonic-stem-(ES)-cell-derived tyrosine hydroxylase-positive (TH+) cells into Parkinsonian mice using behavioral tests and immunohistochemical evaluation.
Methods. Undifferentiated ES cells carrying the enhanced green fluorescent protein (EGFP) gene were differentiated into a cell population containing TH+ neurons using a five-step in vitro differentiation method. These ES-cell-derived cells were used as allografts in Parkinsonian mice, made by administering injections of 6-hydroxydopamine (6-OHDA). Fifteen hemiparkinsonian mice were divided into three groups. Four weeks after 6-OHDA injection, mice in groups 1, 2, and 3 received phosphate-buffered saline, 1 x 104 graft cells, and 1 x 105 graft cells, respectively, into their dopamine-denervated striata.
Results. Improved rotational behavior was observed in the graft-transplanted groups (groups 2 and 3) 2 weeks after transplantation. Mice in group 2 displayed a continuous maintenance of reduced rotational behavior, while those in group 3 showed ipsilateral rotation toward the lesioned side at 4, 6, and 8 weeks after transplantation. Tumor formation was observed in one mouse in group 3. TH+ cells were found at the grafted sites 8 weeks after transplantation in mice in groups 2 and 3, some of which were immunopositive to GFP, demonstrating the presence of dopaminergic neurons derived from the ES cells.
Conclusion. Transplantation of in vitro differentiated ES cells changed rotational behavior in Parkinsonian mice. Our results suggest the potential availability of ES cells for Parkinson’s disease.
This article has been cited by other articles:
|
|
 |
|
  W. Murrell, A. Wetzig, M. Donnellan, F. Feron, T. Burne, A. Meedeniya, J. Kesby, J. Bianco, C. Perry, P. Silburn, et al. Olfactory Mucosa Is a Potential Source for Autologous Stem Cell Therapy for Parkinson's Disease Stem Cells, August 1, 2008; 26(8): 2183 - 2192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Thomas, S. Friedman, and R.-Y. Lin Thyroid stem cells: lessons from normal development and thyroid cancer Endocr. Relat. Cancer, March 1, 2008; 15(1): 51 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Cesnulevicius, M. Timmer, M. Wesemann, T. Thomas, T. Barkhausen, and C. Grothe Nucleofection Is the Most Efficient Nonviral Transfection Method for Neuronal Stem Cells Derived from Ventral Mesencephali with No Changes in Cell Composition or Dopaminergic Fate Stem Cells, December 1, 2006; 24(12): 2776 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Lamba, M. O. Karl, C. B. Ware, and T. A. Reh Efficient generation of retinal progenitor cells from human embryonic stem cells PNAS, August 22, 2006; 103(34): 12769 - 12774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Arufe, M. Lu, A. Kubo, G. Keller, T. F. Davies, and R.-Y. Lin Directed Differentiation of Mouse Embryonic Stem Cells into Thyroid Follicular Cells Endocrinology, June 1, 2006; 147(6): 3007 - 3015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fukuda, J. Takahashi, K. Watanabe, H. Hayashi, A. Morizane, M. Koyanagi, Y. Sasai, and N. Hashimoto Fluorescence-Activated Cell Sorting-Based Purification of Embryonic Stem Cell-Derived Neural Precursors Averts Tumor Formation after Transplantation Stem Cells, March 1, 2006; 24(3): 763 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Weiss, S. Medicetty, A. R. Bledsoe, R. S. Rachakatla, M. Choi, S. Merchav, Y. Luo, M. S. Rao, G. Velagaleti, and D. Troyer Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson's Disease Stem Cells, March 1, 2006; 24(3): 781 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Martinat, J.-J. Bacci, T. Leete, J. Kim, W. B. Vanti, A. H. Newman, J. H. Cha, U. Gether, H. Wang, and A. Abeliovich Cooperative transcription activation by Nurr1 and Pitx3 induces embryonic stem cell maturation to the midbrain dopamine neuron phenotype PNAS, February 21, 2006; 103(8): 2874 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Fu, Y.-C. Cheng, M.-Y. A. Lin, H. Cheng, P.-M. Chu, S.-C. Chou, Y.-H. Shih, M.-H. Ko, and M.-S. Sung Conversion of Human Umbilical Cord Mesenchymal Stem Cells in Wharton's Jelly to Dopaminergic Neurons In Vitro: Potential Therapeutic Application for Parkinsonism Stem Cells, January 1, 2006; 24(1): 115 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. -L. Kuan and R. A. Barker New Therapeutic Approaches to Parkinson's Disease Including Neural Transplants Neurorehabil Neural Repair, September 1, 2005; 19(3): 155 - 181. [Abstract] [PDF]
|
|
|
|
|
|
K. J. Cho, K. A. Trzaska, S. J. Greco, J. McArdle, F. S. Wang, J.-H. Ye, and P. Rameshwar Neurons Derived From Human Mesenchymal Stem Cells Show Synaptic Transmission and Can Be Induced to Produce the Neurotransmitter Substance P by Interleukin-1{alpha} Stem Cells, March 1, 2005; 23(3): 383 - 391. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
