| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Whitehead Institute, Cambridge, Massachusetts, USA;
b ViaCell, Inc., Cambridge, Massachusetts, USA;
c Division of Hematology/Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
Key Words. Embryonic stem cells • Hemangioblast • Thrombopoietin • c-Mpl
George Q. Daley, M.D., Ph.D., Whitehead Institute, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA. Phone: 617-258-7209; Fax: 617-258-5213; e-mail: daley{at}wi.mit.edu
Vascular endothelial growth factor (VEGF) and stem cell factor (SCF) act as growth factors for the hemangioblast, an embryonic progenitor of the hematopoietic and endothelial lineages. Because thrombopoietin (TPO) and its receptor, c-Mpl, regulate primitive hematopoietic populations, including bone marrow hematopoietic stem cells, we investigated whether TPO acts on the hemangioblasts that derive from differentiation of embryonic stem cells in vitro. Reverse transcriptase polymerase chain reaction analysis detected expression of c-Mpl beginning on day 3 of embryoid body differentiation when the hemangioblast first arises. In assays of the hemangioblast colony-forming cell (BL-CFC), TPO alone supported BL-CFC formation and nearly doubled the number of BL-CFC when added together with VEGF and SCF. When replated under the appropriate conditions, TPO-stimulated BL-CFC gave rise to secondary hematopoietic colonies, as well as endothelial cells, confirming their nature as hemangioblasts. Addition of a neutralizing anti-VEGF antibody did not block TPO enhancement of BL-CFC formation, suggesting that TPO acts independently of VEGF. These results establish that Mpl signaling plays a role in the earliest stages of hematopoietic development and that TPO represents a third growth factor influencing hemangioblast formation.
This article has been cited by other articles:
|
|
 |
|
  L. Petit-Cocault, C. Volle-Challier, M. Fleury, B. Peault, and M. Souyri Dual role of Mpl receptor during the establishment of definitive hematopoiesis Development, August 15, 2007; 134(16): 3031 - 3040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. R. Perlingeiro Endoglin is required for hemangioblast and early hematopoietic development Development, August 15, 2007; 134(16): 3041 - 3048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. LENGERKE, S. MCKINNEY-FREEMAN, O. NAVEIRAS, F. YATES, Y. WANG, D. BANSAL, and G. Q. DALEY The Cdx-Hox Pathway in Hematopoietic Stem Cell Formation from Embryonic Stem Cells Ann. N.Y. Acad. Sci., June 1, 2007; 1106(1): 197 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yao, B. Liu, X. Wang, Y. Lan, N. Hou, X. Yang, and N. Mao Identification of High Proliferative Potential Precursors with Hemangioblastic Activity in the Mouse Aorta-Gonad- Mesonephros Region Stem Cells, June 1, 2007; 25(6): 1423 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-M. Zou, M.-H. Luo, A. Reed, M. R. Kelley, and M. C. Yoder Ape1 regulates hematopoietic differentiation of embryonic stem cells through its redox functional domain Blood, March 1, 2007; 109(5): 1917 - 1922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tober, A. Koniski, K. E. McGrath, R. Vemishetti, R. Emerson, K. K. L. de Mesy-Bentley, R. Waugh, and J. Palis The megakaryocyte lineage originates from hemangioblast precursors and is an integral component both of primitive and of definitive hematopoiesis Blood, February 15, 2007; 109(4): 1433 - 1441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Priddle, D. R. E. Jones, P. W. Burridge, and R. Patient Hematopoiesis from Human Embryonic Stem Cells: Overcoming the Immune Barrier in Stem Cell Therapies Stem Cells, April 1, 2006; 24(4): 815 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, F. Yates, O. Naveiras, P. Ernst, and G. Q. Daley Embryonic stem cell-derived hematopoietic stem cells PNAS, December 27, 2005; 102(52): 19081 - 19086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Amano, N. R. Hackett, S. Rafii, and R. G. Crystal Thrombopoietin Gene Transfer-Mediated Enhancement of Angiogenic Responses to Acute Ischemia Circ. Res., August 19, 2005; 97(4): 337 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Chute, G. G. Muramoto, J. Fung, and C. Oxford Soluble factors elaborated by human brain endothelial cells induce the concomitant expansion of purified human BM CD34+CD38- cells and SCID-repopulating cells Blood, January 15, 2005; 105(2): 576 - 583. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
