| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Cell Biology, Beijing Institute of Basic Medical Sciences, Beijing, China;
b Department of Urology, PLA General Hospital, Beijing, China.
Key Words. Mesenchymal progenitor cells • Mixed leukocyte reaction • Immune regulation
Ning Mao, M.D., Department of Cell Biology, Beijing Institute of Basic Medical Sciences, 27 Tai-ping Road, Beijing 100850, People’s Republic of China. Telephone: 86-10-66931320; Fax: 86-10-68213039; e-mail: maoning{at}nic.bmi.ac.cn or maoning2002{at}hotmail.com
Bone marrow contains a population of rare progenitor cells capable of differentiating into osteoblasts, chondrocytes, adipocytes, myoblasts, and hematopoiesis-supporting stromal cells. These cells, referred to as mesenchymal progenitor cells (MPCs), can be purified and culture-expanded from animals and humans. Using bone-marrow-conditioned medium combined with basic fibroblast growth factor, we cultured a relatively homogeneous population of MPCs from murine bone marrow, which uniformly expressed stem cell antigen-1, CD29, CD44, c-kit, and CD105, while being negative for expression of CD45, CD31, and CD34. In vitro differentiation assays showed the tripotential differentiation capacities of these cells toward adipogenic, osteogenic, and chondrogenic lineages. Most importantly, immunophenotypic analyses demonstrated that MPCs did not express major histocompatibility complex class II molecules or the T-cell costimulatory molecules CD80 and CD86, consistent with further investigation showing that MPCs failed to elicit a proliferative response from allogeneic lymphocytes. Moreover, when allogeneic or third-party MPCs were added to T cells stimulated by allogeneic lymphocytes or the potent T-cell mitogen concanavalin-A, a significant reduction in T-cell proliferation was observed. In conclusion, our data demonstrate that we successfully isolated and culture-expanded a relatively homogeneous population of MPCs from adult murine bone marrow. Additionally, these primary cells could suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. This immunoregulatory feature of MPCs strongly implies that they may have potential applications in allograft transplantation.
This article has been cited by other articles:
|
|
 |
|
  X.-Y. Wang, Y. Lan, W.-Y. He, L. Zhang, H.-Y. Yao, C.-M. Hou, Y. Tong, Y.-L. Liu, G. Yang, X.-D. Liu, et al. Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos Blood, February 15, 2008; 111(4): 2436 - 2443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Li, X. Wang, and C. Niyibizi Distribution of Single-Cell Expanded Marrow Derived Progenitors in a Developing Mouse Model of Osteogenesis Imperfecta Following Systemic Transplantation Stem Cells, December 1, 2007; 25(12): 3183 - 3193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Holmes and W. L. Stanford Concise Review: Stem Cell Antigen-1: Expression, Function, and Enigma Stem Cells, June 1, 2007; 25(6): 1339 - 1347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Koide, S. Morikawa, Y. Mabuchi, Y. Muguruma, E. Hiratsu, K. Hasegawa, M. Kobayashi, K. Ando, K. Kinjo, H. Okano, et al. Two Distinct Stem Cell Lineages in Murine Bone Marrow Stem Cells, May 1, 2007; 25(5): 1213 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inoue, S. Noda, N. Ogonuki, H. Miki, S. Inoue, K. Katayama, K. Mekada, H. Miyoshi, and A. Ogura Differential Developmental Ability of Embryos Cloned from Tissue-Specific Stem Cells Stem Cells, May 1, 2007; 25(5): 1279 - 1285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Anversa, J. Kajstura, and A. Leri If I Can Stop One Heart From Breaking Circulation, February 20, 2007; 115(7): 829 - 832. [Full Text] [PDF]
|
|
|
|
|
|
P. Tropel, N. Platet, J.-C. Platel, D. Noel, M. Albrieux, A.-L. Benabid, and F. Berger Functional Neuronal Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells, December 1, 2006; 24(12): 2868 - 2876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Riggi, L. Cironi, P. Provero, M.-L. Suva, J.-C. Stehle, K. Baumer, L. Guillou, and I. Stamenkovic Expression of the FUS-CHOP Fusion Protein in Primary Mesenchymal Progenitor Cells Gives Rise to a Model of Myxoid Liposarcoma. Cancer Res., July 15, 2006; 66(14): 7016 - 7023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Guo, H. Li, X. Li, X. Yu, H. Wang, P. Tang, and N. Mao In Vitro Characteristics and In Vivo Immunosuppressive Activity of Compact Bone-Derived Murine Mesenchymal Progenitor Cells Stem Cells, April 1, 2006; 24(4): 992 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Deng, B. E. Petersen, D. A. Steindler, M. L. Jorgensen, and E. D. Laywell Mesenchymal Stem Cells Spontaneously Express Neural Proteins in Culture and Are Neurogenic after Transplantation Stem Cells, April 1, 2006; 24(4): 1054 - 1064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Phinney, K. Hill, C. Michelson, M. DuTreil, C. Hughes, S. Humphries, R. Wilkinson, M. Baddoo, and E. Bayly Biological Activities Encoded by the Murine Mesenchymal Stem Cell Transcriptome Provide a Basis for Their Developmental Potential and Broad Therapeutic Efficacy Stem Cells, January 1, 2006; 24(1): 186 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Riggi, L. Cironi, P. Provero, M.-L. Suva, K. Kaloulis, C. Garcia-Echeverria, F. Hoffmann, A. Trumpp, and I. Stamenkovic Development of Ewing's Sarcoma from Primary Bone Marrow-Derived Mesenchymal Progenitor Cells Cancer Res., December 15, 2005; 65(24): 11459 - 11468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Leri, J. Kajstura, and P. Anversa Cardiac Stem Cells and Mechanisms of Myocardial Regeneration Physiol Rev, October 1, 2005; 85(4): 1373 - 1416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Ehrlich, H. Y. Chung, I. Ghobrial, S. J. Choi, F. Morandi, S. Colla, V. Rizzoli, G. D. Roodman, and N. Giuliani IL-3 is a potential inhibitor of osteoblast differentiation in multiple myeloma Blood, August 15, 2005; 106(4): 1407 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-X. Jiang, Y. Zhang, B. Liu, S.-X. Zhang, Y. Wu, X.-D. Yu, and N. Mao Human mesenchymal stem cells inhibit differentiation and function of monocyte-derived dendritic cells Blood, May 15, 2005; 105(10): 4120 - 4126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gondo, T. Yanase, T. Okabe, T. Tanaka, H. Morinaga, M. Nomura, K. Goto, and H. Nawata SF-1/Ad4BP transforms primary long-term cultured bone marrow cells into ACTH-responsive steroidogenic cells Genes Cells, December 1, 2004; 9(12): 1239 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Sorkin, K. C. Dee, and M. L. Knothe Tate "Culture shock" from the bone cell's perspective: emulating physiological conditions for mechanobiological investigations Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1527 - C1536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-W. D. Leong, F.-T. Chew, and D. W. Hutmacher Isolating Bone Marrow Stem Cells Using Sieve Technology Stem Cells, November 1, 2004; 22(6): 1123 - 1125. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
