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a Departments of Hematology/Oncology and
c Neurosurgery, Freiburg University Medical Center, Freiburg, Germany;
b Cell Genix Technologie Transfer GmbH, Freiburg, Germany
Key Words. Tumor angiogenesis • Bone marrow transplantation
Alexandros Spyridonidis, M.D., Freiburg University Medical Center, Hugstetterstrasse 55, 79106 Freiburg, Germany. Telephone: 49-761-2703364; Fax: 49-761-2703660; e-mail: spyridonidis{at}mm11.ukl.uni-freiburg.de
Transplanted bone marrow-derived (BM) cells have been shown to home into the tumor vessels of s.c. implanted tumor models and to functionally contribute to tumor neoangiogenesis and tumor growth. However, whether BM cells contribute to the vessels of in situ developing tumors remains unknown. We have taken advantage of the in situ generation of mammary tumors in transgenic mice carrying the polyoma virus middle T oncogene (MMTV-PyVT) to determine whether transplanted BM cells home to and incorporate into the intratumoral vessels. Unfractionated BM from lacZ+ROSA 26 mice was used to rescue irradiated MMTV-PyVT transgenic mice or their wild-type congenics. All transgenic mice were sacrificed when they developed easily palpable mammary tumors. BM cells recruited and incorporated into the vasculature were identified by coexpression of lacZ and CD31, evidence that these cells had a distinctive, elongated appearance and that they lined the vessel structures. We found that BM cells home to and incorporate into 1.3% of the vessels of all in situ generated mammary adenocarcinomas examined (n = 8). In contrast, BM cells did not recruit into the vessels of colon or liver of the tumor-bearing mice. Whether these cells contribute to new vessel formation via vasculogenesis or angiogenesis or simply attach to, and integrate into, the growing tips or shafts of pre-existing vessels has to be determined. BM could be used as a vehicle for the specific transport of antiangiogenic signals into the tumor vascular bed.
This article has been cited by other articles:
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  G. C. Schatteman, M. Dunnwald, and C. Jiao Biology of bone marrow-derived endothelial cell precursors Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H1 - H18. [Abstract] [Full Text] [PDF]
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 H. Sugimoto, T. M. Mundel, M. Sund, L. Xie, D. Cosgrove, and R. Kalluri Bone-marrow-derived stem cells repair basement membrane collagen defects and reverse genetic kidney disease PNAS, May 9, 2006; 103(19): 7321 - 7326. [Abstract] [Full Text] [PDF]
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