HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miyamoto, K. Articles by Ito, Y. Search for Related Content PubMed PubMed Citation Articles by Miyamoto, K. Articles by Ito, Y.

Human Placenta Feeder Layers Support Undifferentiated Growth of Primate Embryonic Stem Cells

^a Tokyo Metropolitan Institute of Technology, Systems Engineering Science, Hino, Tokyo, Japan;
^b Okayama University Graduate School of Medicine and Dentistry, Department of Pathology, Okayama, Okayama, Japan;
^c Hino Municipal Hospital, Hino, Tokyo, Japan;
^d Kibi International University, Health Science, Takahashi, Okayama, Japan;
^e Kanagawa Academy of Science and Technology, Kawasaki, Kanagawa, Japan

Key Words. Primate ES cells • Feeder layer • Human placenta • Teratoma

Correspondence: Kanji Miyamoto, Ph.D., Department of Systems Engineering Science, Tokyo Metropolitan Institute of Technology, Asahigaoka 6-6, Hino, Tokyo 191-0065, Japan. Telephone: 81-42-585-8641; Fax: 81-42-585-8641; e-mail: kmiyamot{at}cc.tmit.ac.jp

Various undifferentiated embryonic stem (ES) cells can grow on mouse embryonic fibroblast (MEF) feeders. However, the risk of zoonosis from animal feeders to human ES cells generally excludes the clinical use of these human ES cells. We have found that human placenta is a useful source of feeder cells for the undifferentiated growth of primate ES cells. As on MEF feeders, primate ES cells cultured on human amniotic epithelial (HAE) feeder cells and human chorionic plate (HCP) cells had undifferentiated growth. The cultured primate ES cells expressed Oct-4, alkaline phosphatase, and SSEA-4. The primate ES cells on HAE feeder cells produced typical immature teratomas in vivo after injection into severe combined immunodeficient mice. Human placenta is quite novel and important because it would provide a relatively available source of feeders for the growth of human ES cells for therapeutic purposes that are also free of ethical complications.

This article has been cited by other articles:

				S. Ilancheran, A. Michalska, G. Peh, E. M Wallace, M. Pera, and U. Manuelpillai Stem Cells Derived from Human Fetal Membranes Display Multilineage Differentiation Potential Biol Reprod, September 1, 2007; 77(3): 577 - 588. [Abstract] [Full Text] [PDF]

				Y.-T. Chen, W. Li, Y. Hayashida, H. He, S.-Y. Chen, D. Y. Tseng, A. Kheirkhah, and S. C. G. Tseng Human Amniotic Epithelial Cells as Novel Feeder Layers for Promoting Ex Vivo Expansion of Limbal Epithelial Progenitor Cells Stem Cells, August 1, 2007; 25(8): 1995 - 2005. [Abstract] [Full Text] [PDF]

				W. Li, H. He, C.-L. Kuo, Y. Gao, T. Kawakita, and S. C. G. Tseng Basement membrane dissolution and reassembly by limbal corneal epithelial cells expanded on amniotic membrane. Invest. Ophthalmol. Vis. Sci., June 1, 2006; 47(6): 2381 - 2389. [Abstract] [Full Text] [PDF]

				T. Miki, T. Lehmann, H. Cai, D. B. Stolz, and S. C. Strom Stem Cell Characteristics of Amniotic Epithelial Cells Stem Cells, October 1, 2005; 23(10): 1549 - 1559. [Abstract] [Full Text] [PDF]

				Q. Wang, Z. F. Fang, F. Jin, Y. Lu, H. Gai, and H. Z. Sheng Derivation and Growing Human Embryonic Stem Cells on Feeders Derived from Themselves Stem Cells, September 1, 2005; 23(9): 1221 - 1227. [Abstract] [Full Text] [PDF]