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Differential Effects of Culture Conditions on the Migration Pattern of Stromal Cell–Derived Factor–Stimulated Hematopoietic Stem Cells

^a Institute of Immunology and
^b Department of Gynecology, Marienhospital, University of Witten/Herdecke, Witten, Germany

Key Words. SDF-1 • Ex vivo expansion • Cell migration • Cytokines • Hematopoietic stem cells

Correspondence: Corinna Weidt, Institute of Immunology, University of Witten/Herdecke, Stockumer Str. 10, 58448 Witten, Germany. Telephone: 49-2302-669-152; Fax:49-2302-669-158; e-mail: cweidt{at}uni-wh.de

Evidence is mounting that hematopoietic stem cells (HSCs) play a critical role in bone marrow regeneration and tissue renewal, for which migration is an obvious prerequisite. Computer-aided analysis and a three-dimensional collagen matrix assay enabled us to analyze single-cell migratory characteristics of stromal cell–derived factor-1 (SDF-1)–stimulated cord blood–derived HSCs. We defined and resolved specific migratory parameters in spontaneous and SDF-1–induced migration of these cells. The addition of interleukin 6 to the culture medium led to differential SDF-1–stimulated migratory response, which comprised a recruitment of nonmoving cells and an increase in speed and frequency of pauses but a decrease in pause duration. We were thus able to decipher the exact parameters that result in an increase in the migration of HSCs and demonstrate that extensive analysis of single-cell behavior is elementary in the study of stem cell migration.

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