HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vas, V. Articles by Uher, F. Search for Related Content PubMed PubMed Citation Articles by Vas, V. Articles by Uher, F.

Biphasic Effect of Recombinant Galectin-1 on the Growth and Death of Early Hematopoietic Cells

^a Stem Cell Biology, National Medical Center, Budapest, Hungary;
^b Lymphocyte Signal Transduction Laboratory, Institute of Genetics, Biological Research Center of Hungarian Academy of Sciences, Szeged, Hungary;
^c Polyclinic of Hospitaller Brothers of St. John of God, Budapest, Hungary

Key Words. Apoptosis • Cobblestone area–forming cells • Galectin-1 Hematopoietic stem and progenitor cells • Human • Mouse

Correspondence: Ferenc Uher, Ph.D., National Medical Center, Stem Cell Biology, Diószegi ut 64., Budapest, Hungary, H-1113. Telephone: 36-1-372-4334; Fax: 36-1-372-4352; e-mail: uher{at}ohvi.hu

Galectin-1 is a member of the family of ß-galactoside binding animal lectins, galectins. Its presence in the bone marrow has been detected; however, its role in the regulation of hematopoiesis is unknown. In the present study, we have evaluated the effect of recombinant human galectin-1 on the proliferation and survival of murine and human hematopoietic stem and progenitor cells. We show that low amount of galectin-1 (10 ng/ml) increases the formation of granulocyte-macrophage and erythroid colonies and the frequencies of day-7 cobblestone area–forming cells on a lactose-inhibitable fashion. In contrast, high amount of galectin-1 (10 µg/ml) dramatically reduces the growth of the committed blood-forming progenitor cells as well as the much younger, lineage-negative hematopoietic cells (day-28 to -35 cobblestone area–forming cells). This inhibition is not blocked by lactose and, therefore, is largely independent of the ß-galactoside–binding site of the lectin. Furthermore, assays to detect apoptosis render it likely that the high amount of galectin-1 acts as a classical proapoptotic factor for the premature hematopoietic cells.

This article has been cited by other articles:

				F. Benvenuto, S. Ferrari, E. Gerdoni, F. Gualandi, F. Frassoni, V. Pistoia, G. Mancardi, and A. Uccelli Human Mesenchymal Stem Cells Promote Survival of T Cells in a Quiescent State Stem Cells, July 1, 2007; 25(7): 1753 - 1760. [Abstract] [Full Text] [PDF]

				S. R. Stowell, S. Karmakar, C. J. Stowell, M. Dias-Baruffi, R. P. McEver, and R. D. Cummings Human galectin-1, -2, and -4 induce surface exposure of phosphatidylserine in activated human neutrophils but not in activated T cells Blood, January 1, 2007; 109(1): 219 - 227. [Abstract] [Full Text] [PDF]

				I. Camby, M. Le Mercier, F. Lefranc, and R. Kiss Galectin-1: a small protein with major functions Glycobiology, November 1, 2006; 16(11): 137R - 157R. [Abstract] [Full Text] [PDF]

				V. A. Biron, M. M. Iglesias, M. F. Troncoso, M. Besio-Moreno, Z. J. Patrignani, O. P. Pignataro, and C. Wolfenstein-Todel Galectin-1: biphasic growth regulation of Leydig tumor cells Glycobiology, September 1, 2006; 16(9): 810 - 821. [Abstract] [Full Text] [PDF]

				M. Sakaguchi, T. Shingo, T. Shimazaki, H. J. Okano, M. Shiwa, S. Ishibashi, H. Oguro, M. Ninomiya, T. Kadoya, H. Horie, et al. A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells PNAS, May 2, 2006; 103(18): 7112 - 7117. [Abstract] [Full Text] [PDF]