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Stem Cells 2005;23:315-323 www.StemCells.com
© 2005 AlphaMed Press

Basic Fibroblast Growth Factor Supports Undifferentiated Human Embryonic Stem Cell Growth Without Conditioned Medium

Chunhui Xu, Elen Rosler, Jianjie Jiang, Jane S. Lebkowski, Joseph D. Gold, Chris O’Sullivan, Karen Delavan-Boorsma, Michael Mok, Adrienne Bronstein, Melissa K. Carpenter

Geron Corporation, Menlo Park, California, USA

Key Words. Human embryonic stem cells • Growth factors • Telomerase • Stem cell markers • Differentiation

Correspondence: Chunhui Xu, Ph.D., Geron Corporation, 230 Constitution Drive, Menlo Park, California 94025, USA. Telephone: 650-473-7795; fax: 650-473-7750; e-mail: cxu{at}geron.com

Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor (bFGF), stem cell factor (SCF), and fetal liver tyrosine kinase-3 ligand (Flt3L), we evaluated these and other growth factors for their ability to maintain undifferentiated hESCs in the absence of conditioned medium. We found cultures maintained in bFGF alone or in combination with other factors showed characteristics similar to MEF-CM control cultures, including morphology, surface marker and transcription factor expression, telomerase activity, differentiation, and karyotypic stability. In contrast, cells in media containing Flt-3L, thrombopoietin, and SCF, individually or in combination, showed almost complete differentiation after 6 weeks in culture. These data demonstrate that hESCs can be maintained in nonconditioned medium using growth factors.




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