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Characterization of Ionic Currents in Human Mesenchymal Stem Cells from Bone Marrow

Department of Medicine, Research Centre on Heart, Brain, Hormones and Healthy Aging, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China

Key Words. Ca²⁺-activated K⁺ current • Heag K⁺ current • Human mesenchymal stem cells • Ion channels • Transient outward K⁺ current • TTX-sensitive Na⁺ current

Correspondence: Gui-Rong Li, L8–01, Laboratory Block, Faculty of Medicine Building, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China. Telephone: 852-2819-2830; Fax: 852-2816-2095; e-mail: grli{at}hkucc.hku.hk

This study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca²⁺-activated K⁺ channel (I_KCa) blocker iberiotoxin, a transient outward K⁺ current (I_to) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K⁺ current (IK_DR)-like ether-à-go-go (eag) K⁺ channel. In addition, tetrodotoxin-sensitive sodium current (I_Na.TTX) and nifedipine-sensitive L-type Ca²⁺ current (I_Ca.L) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for I_KCa, Kv4.2 and Kv1.4 for I_to, heag1 for IK_DR, hNE-Na for I_Na.TTX, and CACNAIC for I_Ca.L. These results demonstrate that multiple functional ion channel currents—that is, I_KCa, I_to, heag1, I_Na.TTX, and I_Ca.L—are expressed in hMSCs from bone marrow.

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