| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Biological Sciences, Biochemistry Section, University of Modena and Reggio Emilia, Modena, Italy;
b Institute of Hematology and Medical Oncology "L. & A. Seragnoli," University of Bologna, Italy
Key Words. CD34+ stem cells • CD34– • Hematopoietic stem cells • Microarray • Proliferation • Self-renewal • Differentiation • Engraftment
Correspondence: Sergio Ferrari, M.D., Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Università di Modena e Reggio Emilia, Via Campi 287, 41100 Modena, Italy. Telephone: 39-059-2055400; Fax: 39-059-2055410; e-mail: ferrari.sergio{at}unimo.it
The gene expression profile of CD34– hematopoietic stem cells (HSCs) and the correlations with their biological properties are still poorly understood. To address this issue, we used the DNA microarray technology to compare the expression profiles of different peripheral blood hemopoietic stem/progenitor cell subsets, lineage-negative (Lin–) CD34–, Lin–CD34+, and Lin+CD34+ cells. The analysis of gene categories differentially expressed shows that the expression of CD34 is associated with cell cycle entry and metabolic activation, such as DNA, RNA, and protein synthesis. Moreover, the significant upregulation in CD34– cells of pathways inhibiting HSC proliferation induces a strong differential expression of cyclins, cyclin-dependent kinases (CDKs), CDK inhibitors, and growth-arrest genes. According to the expression of their receptors and transducers, interleukin (IL)-10 and IL-17 showed an inhibitory effect on the clonogenic activity of CD34– cells. Conversely, CD34+ cells were sensitive to the mitogenic stimulus of thrombopoietin. Furthermore, CD34– cells express preferentially genes related to neural, epithelial, and muscle differentiation. The analysis of transcription factor expression shows that the CD34 induction results in the upregulation of genes related to self-renewal and lineage commitment. The preferential expression in CD34+ cells of genes supporting the HSC mobilization and homing to the bone marrow, such as chemokine receptors and integrins, gives the molecular basis for the higher engraftment capacity of CD34+ cells. Thus, the different kinetic status of CD34– and CD34+ cells, detailed by molecular and functional analysis, significantly influences their biological behavior.
This article has been cited by other articles:
|
|
 |
|
  S. Rutella, G. Bonanno, A. Procoli, A. Mariotti, M. Corallo, M. G. Prisco, A. Eramo, C. Napoletano, D. Gallo, A. Perillo, et al. Cells with Characteristics of Cancer Stem/Progenitor Cells Express the CD133 Antigen in Human Endometrial Tumors Clin. Cancer Res., July 1, 2009; 15(13): 4299 - 4311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Salati, R. Zini, E. Bianchi, A. Testa, F. Mavilio, R. Manfredini, and S. Ferrari Role of CD34 Antigen in Myeloid Differentiation of Human Hematopoietic Progenitor Cells Stem Cells, April 1, 2008; 26(4): 950 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Salati, E. Bianchi, R. Zini, E. Tenedini, D. Quaglino, R. Manfredini, and S. Ferrari Eosinophils, but not neutrophils, exhibit an efficient DNA repair machinery and high nucleolar activity Haematologica, October 1, 2007; 92(10): 1311 - 1318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Rossi, R. Manfredini, F. Bertolini, D. Ferrari, M. Fogli, R. Zini, S. Salati, V. Salvestrini, S. Gulinelli, E. Adinolfi, et al. The extracellular nucleotide UTP is a potent inducer of hematopoietic stem cell migration Blood, January 15, 2007; 109(2): 533 - 542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Guglielmelli, R. Zini, C. Bogani, S. Salati, A. Pancrazzi, E. Bianchi, F. Mannelli, S. Ferrari, M.-C. Le Bousse-Kerdiles, A. Bosi, et al. Molecular Profiling of CD34+ Cells in Idiopathic Myelofibrosis Identifies a Set of Disease-Associated Genes and Reveals the Clinical Significance of Wilms' Tumor Gene 1 (WT1) Stem Cells, January 1, 2007; 25(1): 165 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Keller, S. Addya, R. Vadigepalli, B. Banini, K. Delgrosso, H. Huang, and S. Surrey Transcriptional regulatory network analysis of developing human erythroid progenitors reveals patterns of coregulation and potential transcriptional regulators Physiol Genomics, December 13, 2006; 28(1): 114 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Lapidot, A. Dar, and O. Kollet How do stem cells find their way home? Blood, September 15, 2005; 106(6): 1901 - 1910. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
