| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Ophthalmology, University of Aberdeen, Scotland, United Kingdom;
b Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Austria
Key Words. Cornea • CD34 • Hemopoietic stem cell • Dendritic cell • Leukocytes
Correspondence: John V. Forrester, M.D., Department of Ophthalmology, University of Aberdeen, AB255ZD, Aberdeen, Scotland, United Kingdom. Telephone: 0044-122-455-3782; Fax: 0044-122-455-5955; e-mail: j.forrester{at}abdn.ac.uk
Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, phenotype, and distribution. Single-cell suspensions from normal mouse corneas were also prepared and analyzed by flow cytometry. After short-term culture of corneal stromal explants, nonadherent cells were harvested and cytospins were prepared and stained for different markers.
Combined staining for F-actin and leukocyte differentiation markers clearly showed that the corneal stroma contains a population of CD45+ resident bone marrow–derived cells, whereas most cells were CD45-F-actin+ keratocytes. A significant proportion (two thirds) of CD45+ cells in the normal corneal stroma expressed CD34+, whereas no CD45– cells (i.e., keratocytes) coexpressed CD34. In addition, CD34+ cells were CD11c– and CD11b+. Fewer than 10% of the CD34+ cells also coexpressed Sca-1+, but no CD34+ cells coexpressed major histocompatibility complex (MHC) class II+. In contrast, the remaining population of CD45+CD34– cells in the corneal stroma expressed CD11b, MHC class II+ but not CD11c and were found mostly in the anterior and peripheral part of stroma. These cells are in intimate contact with corneal keratocytes, which stained only for F-actin and were negative for all leukocyte markers. Very few CD45+ cells expressed the B220 marker, suggesting a plasmacytoid dendritic cell phenotype. Flow cytometry analyses confirmed the morphometric data showing that 68% of CD45+ cells coexpress CD34 and CD11b, whereas 22% are CD11b+CD34–.
We conclude that the normal mouse cornea contains two populations of bone marrow–derived leukocytes, both of which are distinct from stromal keratocytes. The larger population resembles CD34+ hemopoietic stem cells, whereas the smaller population are CD34–CD11b+ MHC class II+ macrophages. A very small percentage comprises plasmacytoid dendritic cells.
This article has been cited by other articles:
|
|
 |
|
  H. R. Chinnery, E. Pearlman, and P. G. McMenamin Cutting Edge: Membrane Nanotubes In Vivo: A Feature of MHC Class II+ Cells in the Mouse Cornea J. Immunol., May 1, 2008; 180(9): 5779 - 5783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kuffova, M. Netukova, L. Duncan, A. Porter, B. Stockinger, and J. V. Forrester Cross Presentation of Antigen on MHC Class II via the Draining Lymph Node after Corneal Transplantation in Mice J. Immunol., February 1, 2008; 180(3): 1353 - 1361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gillette-Ferguson, K. Daehnel, A. G. Hise, Y. Sun, E. Carlson, E. Diaconu, H. F. McGarry, M. J. Taylor, and E. Pearlman Toll-Like Receptor 2 Regulates CXC Chemokine Production and Neutrophil Recruitment to the Cornea in Onchocerca volvulus/ Wolbachia-Induced Keratitis Infect. Immun., December 1, 2007; 75(12): 5908 - 5915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Chinnery, M. J. Ruitenberg, G. W. Plant, E. Pearlman, S. Jung, and P. G. McMenamin The Chemokine Receptor CX3CR1 Mediates Homing of MHC class II-Positive Cells to the Normal Mouse Corneal Epithelium Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1568 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A Shanmuganathan, T. Foster, B. B Kulkarni, A. Hopkinson, T. Gray, D. G Powe, J. Lowe, and H. S Dua Morphological characteristics of the limbal epithelial crypt Br. J. Ophthalmol., April 1, 2007; 91(4): 514 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lin, E. Carlson, E. Diaconu, and E. Pearlman CXCL1/KC and CXCL5/LIX are selectively produced by corneal fibroblasts and mediate neutrophil infiltration to the corneal stroma in LPS keratitis J. Leukoc. Biol., March 1, 2007; 81(3): 786 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sosnova-Netukova, P. Kuchynka, and J. V Forrester The suprabasal layer of corneal epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes Br. J. Ophthalmol., March 1, 2007; 91(3): 372 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Perrella, P Brusini, R Spelat, P Hossain, A Hopkinson, and H S Dua Expression of haematopoietic stem cell markers, CD133 and CD34 on human corneal keratocytes Br. J. Ophthalmol., January 1, 2007; 91(1): 94 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yoshida, S. Shimmura, N. Nagoshi, K. Fukuda, Y. Matsuzaki, H. Okano, and K. Tsubota Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea Stem Cells, December 1, 2006; 24(12): 2714 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hori, M. Wang, M. Miyashita, K. Tanemoto, H. Takahashi, T. Takemori, K. Okumura, H. Yagita, and M. Azuma B7-H1-Induced Apoptosis as a Mechanism of Immune Privilege of Corneal Allografts J. Immunol., November 1, 2006; 177(9): 5928 - 5935. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
