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Research Institute of Biotechnology, Histostem Co., Kangdong-Gu, Seoul, Korea
Key Words. Mesenchymal stem cells • Human umbilical cord blood • DNA microarray
Correspondence: Hoeon Kim, Ph.D., Research Institute of Biotechnology, Histostem Co. 518-4 Taijul Bldg., Doonchun-dong, Kangdong-gu, Seoul 134-060, Korea. Telephone: 82-2-488-8154; Fax: 82-2-470-6342; e-mail: hoeonkim{at}seoulcord.co.kr
Mesenchymal stem cells (MSCs) retain both self-renewal and multilineage differentiation capabilities. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the stemness, remain largely unknown. Using high-density oligonucleotide micro-arrays, we obtained the differential gene expression profile between a fraction of mononuclear cells of human umbilical cord blood (UCB) and its MSC subpopulation. Of particular interest was a subset of 47 genes preferentially expressed at 50-fold or higher in MSCs, which could be regarded as a molecular foundation of human MSCs. This subset contains numerous genes encoding collagens, other extracellular matrix or related proteins, cytokines or growth factors, and cytoskeleton-associated proteins but very few genes for membrane and nuclear proteins. In addition, a direct comparison of this microarray-generated transcriptome with the published serial analysis of gene expression data suggests that a molecular context of UCB-derived MSCs is more or less similar to that of bone marrow–derived cells. Altogether, our results will provide a basis for studies on molecular mechanisms controlling core properties of human MSCs.
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