Stem Cells
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online May 11, 2005
Stem Cells Vol. 23 No. 7 August 2005, pp. 895 -902
doi:10.1634/stemcells.2004-0326; www.StemCells.com
© 2005 AlphaMed Press

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-0326v1
2004-0326v2
2004-0326v3
23/7/895    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stojkovic, P.
Right arrow Articles by Stojkovic, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stojkovic, P.
Right arrow Articles by Stojkovic, M.

Human-Serum Matrix Supports Undifferentiated Growth of Human Embryonic Stem Cells

Petra Stojkovica, Majlinda Lakoa, Stefan Przyborskib, Rebecca Stewarta,b, Lyle Armstronga, Jerome Evansc, Xin Zhanga, Miodrag Stojkovica

a Centre for Stem Cell Biology and Developmental Genetics, Institute of Human Genetics, University of Newcastle, Newcastle upon Tyne, United Kingdom;
b School of Biological and Biomedical Sciences, University of Durham, Durham, United Kingdom;
c Institute of Human Genetics, University of Newcastle, Newcastle upon Tyne, United Kingdom

Key Words. Human embryonic stem cells • Pluripotency • Differentiation • Feeder-free

Correspondence: M. Stojkovic, Ph.D., Centre for Stem Cell Biology and Developmental Genetics, Institute of Human Genetics, University of Newcastle, Newcastle upon Tyne, U.K. Telephone: 44-191-241-8638; Fax: 44-191-219-4747; e-mail: miodrag.stojkovic{at}ncl.ac.uk

One of the most frequently used matrices for feeder-free growth of undifferentiated human embryonic stem cells (hESCs) is Matrigel, which supports attachment and growth of undifferentiated hESCs in the presence of mouse embryonic fibroblast–conditioned medium. Unfortunately, application of Matrigel or medium conditioned by mouse embryonic feeder cells is not ideal for potential medical application of hESCs because xenogeneic pathogens can be transmitted through culture conditions. We demonstrate here that human serum as matrix and medium conditioned by differentiated hESCs reduce exposure of hESCs to animal ingredients and provide a safer direction toward completely animal-free conditions for application, handling, and understanding of hESC biology. At the same time, hESCs grown under these conditions maintain all hESC features after prolonged culture, including the developmental potential to differentiate into representative tissues of all three embryonic germ layers, unlimited and undifferentiated proliferative ability, and maintenance of normal karyotype.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Gerecht, J. A. Burdick, L. S. Ferreira, S. A. Townsend, R. Langer, and G. Vunjak-Novakovic
Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells
PNAS, July 3, 2007; 104(27): 11298 - 11303.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
F. Mannello and G. A. Tonti
Concise Review: No Breakthroughs for Human Mesenchymal and Embryonic Stem Cell Culture: Conditioned Medium, Feeder Layer, or Feeder-Free; Medium with Fetal Calf Serum, Human Serum, or Enriched Plasma; Serum-Free, Serum Replacement Nonconditioned Medium, or Ad Hoc Formula? All That Glitters Is Not Gold!
Stem Cells, July 1, 2007; 25(7): 1603 - 1609.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K. Rajala, H. Hakala, S. Panula, S. Aivio, H. Pihlajamaki, R. Suuronen, O. Hovatta, and H. Skottman
Testing of nine different xeno-free culture media for human embryonic stem cell cultures
Hum. Reprod., May 1, 2007; 22(5): 1231 - 1238.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
V.J. Hall, D. Compton, P. Stojkovic, M. Nesbitt, M. Herbert, A. Murdoch, and M. Stojkovic
Developmental competence of human in vitro aged oocytes as host cells for nuclear transfer
Hum. Reprod., January 1, 2007; 22(1): 52 - 62.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
H. Skottman and O. Hovatta
Culture conditions for human embryonic stem cells.
Reproduction, November 1, 2006; 132(5): 691 - 698.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
F. Mannello
Commentary: Multipotent Mesenchymal Stromal Cell Recruitment, Migration, and Differentiation: What Have Matrix Metalloproteinases Got to Do with It?
Stem Cells, August 1, 2006; 24(8): 1904 - 1907.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Lu, R. Hou, C. J. Booth, S.-H. Yang, and M. Snyder
Defined culture conditions of human embryonic stem cells
PNAS, April 11, 2006; 103(15): 5688 - 5693.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. Trounson
The Production and Directed Differentiation of Human Embryonic Stem Cells
Endocr. Rev., April 1, 2006; 27(2): 208 - 219.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS

Copyright © 2005 by AlphaMed Press.