Stem Cells
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First published online June 13, 2005
Stem Cells Vol. 23 No. 8 September 2005, pp. 1044 -1049
doi:10.1634/stemcells.2005-0037; www.StemCells.com
© 2005 AlphaMed Press

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RAPID COMMUNICATIONS

Development of Functional Neurons from Postnatal Stem Cells In Vitro

Eric W. Rowe, Dusan M. Jeftinija, Ksenija Jeftinija, Srdija Jeftinija

Department of Biomedical Sciences, Iowa State University, Ames, Iowa, USA

Key Words. Adult stem cells • Astrocytes • Long-term cultures • Neural stem cell • Calcium transients

Correspondence: Srdija Jeftinija, D.V.M., Ph.D., Department of Biomedical Sciences, Iowa State University, 1098 Vet Med Bldg, Ames, Iowa 50011, USA. Telephone: 515-294-8494; Fax: 515-294-2315; e-mail: sjeftini{at}iastate.edu

In order for stem cells to fulfill their clinical promise, we must understand their developmental transitions and it must be possible to control the differentiation of stem cells into specific cell fates. To understand the mechanism of the sequential restriction and multipotency of stem cells, we have established culture conditions that allow the differentiation of multipotential neural stem cells from postnatal stem cells. We used immunocytochemistry, fluorescence microscopy, and calcium imaging to demonstrate that progeny of adult rat neural stem cells develop into functional neurons that release excitatory neurotransmitters. We also found that the nontoxic heavy chain fragment of tetanus toxin, a toxin that targets neurons with high specificity, retained the specificity toward neural stem cell–derived neurons. These studies show that neural stem cells derived from adult tissues retain the potential to differentiate into functional neurons with morphological and functional properties of mature central nervous system neurons.




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[Abstract] [Full Text] [PDF]


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