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TECHNOLOGY DEVELOPMENT

Establishment of the Gene-Inducible System in Primate Embryonic Stem Cell Lines

^aDepartment of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan;
^bLaboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan;
^cDepartment of Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan

Key Words. Primate embryonic stem cells • Gene-inducible system • Tetracycline • Tet-Off system • Doxycycline Enhanced green fluorescent protein

Correspondence: Hirofumi Suemori, Ph.D., Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shougoin, Sakyo-ku, Kyoto 606-8507, Japan. Telephone: +81-75-751-3821; Fax: +81-75-751-3890; e-mail: hsuemori{at}frontier.kyoto-u.ac.jp

Received December 29, 2005; accepted for publication July 14, 2006.
First published online in STEM CELLS EXPRESS   July 20, 2006.



Human embryonic stem cells (ESCs) would provide a potentially unlimited source for cell replacement therapies. However, the molecular mechanisms involved in the maintenance of "stemness" are not fully understood. Monkey ESCs are much more similar in character to human ESCs than are mouse ESCs. Therefore, studies using monkey ESCs can give conclusions that are more relevant and may be readily applicable to both basic research and clinical applications for future regenerative medicine. For such studies, generation of a gene-inducible system regulatable in primate ESCs would serve as a powerful tool. Here, we established a Tet-Off gene-inducible system in monkey ESC lines. Such manipulated cells maintained ESC characteristics, and inducible gene expression in both the stem cells and differentiated cells could be reliably controlled by doxycycline administration.

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