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OPEN ACCESS ARTICLE
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EMBRYONIC STEM CELLS: CHARACTERIZATION SERIES |
aCentre for Stem Cell Biology and Developmental Genetics, University of Newcastle, Newcastle upon Tyne, United Kingdom;
bCentre for Stem Cell Biology and Regenerative Medicine, School of Biological and Biomedical Science, University of Durham, South Road, Durham, United Kingdom;
cCentro de Investigación Príncipe Felipe, Valencia, Spain
Key Words. Embryo • Human embryonic stem cells • Pluripotent stem cells • Differentiation
Correspondence: Miodrag Stojkovic, Ph.D., Centro de Investigacion Principe Felipe - Cellular Reprogramming Laboratory, C/E.P. Avda. Autopista del Saler 16-3 (junto Oceanografico) Valencia 46013, Spain. Telephone: 381-63-768-6185; e-mail: Sintocell{at}web.de
Received on June 22, 2006;
accepted for publication on September 13, 2006.
First published online in STEM CELLS EXPRESS September 21, 2006.
Human embryonic stem cells (hESC) hold huge promise in modern regenerative medicine, drug discovery, and as a model for studying early human development. However, usage of embryos and derivation of hESC for research and potential medical application has resulted in polarized ethical debates since the process involves destruction of viable developing human embryos. Here we describe that not only developing embryos (morulae and blastocysts) of both good and poor quality but also arrested embryos could be used for the derivation of hESC. Analysis of arrested embryos demonstrated that these embryos express pluripotency marker genes such OCT4, NANOG, and REX1. Derived hESC lines also expressed specific pluripotency markers (TRA-1-60, TRA-1-81, SSEA4, alkaline phosphatase, OCT4, NANOG, TERT, and REX1) and differentiated under in vitro and in vivo conditions into derivates of all three germ layers. All of the new lines, including lines derived from late arrested embryos, have normal karyotypes. These results demonstrate that arrested embryos are additional valuable resources to surplus and donated developing embryos and should be used to study early human development or derive pluripotent hESC.
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