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First published online August 24, 2006
Stem Cells Vol. 24 No. 12 December 2006, pp. 2692 -2702
doi:10.1634/stemcells.2006-0233; www.StemCells.com
© 2006 AlphaMed Press

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STEM CELL GENETICS AND GENOMICS

An Epidermal Neural Crest Stem Cell (EPI-NCSC) Molecular Signature

Yao Fei Hu, Zhi-Jian Zhang, Maya Sieber-Blum

Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, USA

Key Words. Stem cell • Multipotent • Neural crest • Gene profile • Hair follicle • Bulge • Msx2

Correspondence: Maya Sieber-Blum, Ph.D., Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA. Telephone: 414-456-8465; Fax: 414-456-6517; e-mail: sieberbl{at}mcw.edu

Received on April 18, 2006; accepted for publication on August 16, 2006.

First published online in STEM CELLS EXPRESS  August 24, 2006.


Here, we report the first transcriptome for mouse epidermal neural crest stem cells (EPI-NCSC, formerly eNCSCs). In addition, our study resolves conflicting opinions in the literature by showing that EPI-NCSC are distinct from other types of skin-resident stem cells/progenitors. Finally, with the three gene profiles, we have established a foundation and provide a valuable resource for future mouse NCSC research. EPI-NCSC represent a novel type of multipotent adult stem cell that originates from the embryonic neural crest and resides in the bulge of hair follicles. We performed gene profiling by LongSAGE (long serial analysis of gene expression) with mRNA from EPI-NCSC, embryonic NCSC, and in vitro differentiated embryonic neural crest progeny. We have identified important differentially expressed genes, including novel genes and disease genes. Furthermore, using stringent criteria, we have defined an NCSC molecular signature that consists of a panel of 19 genes and is representative of both EPI-NCSC and NCSC. EPI-NCSC have characteristics that combine advantages of embryonic and adult stem cells. Similar to embryonic stem cells, EPI-NCSC have a high degree of innate plasticity, they can be isolated at high levels of purity, and they can be expanded in vitro. Similar to other types of adult stem cell, EPI-NCSC are readily accessible by minimal invasive procedure. Multipotent adult mammalian stem cells are of great interest because of their potential value in future cell replacement therapy by autologous transplantation, which avoids graft rejection.




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S. Thomas, M. Thomas, P. Wincker, C. Babarit, P. Xu, M. C. Speer, A. Munnich, S. Lyonnet, M. Vekemans, and H. C. Etchevers
Human neural crest cells display molecular and phenotypic hallmarks of stem cells
Hum. Mol. Genet., November 1, 2008; 17(21): 3411 - 3425.
[Abstract] [Full Text] [PDF]


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