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First published online December 1, 2005
Stem Cells Vol. 24 No. 2 February 2006, pp. 376 -385
doi:10.1634/stemcells.2005-0234; www.StemCells.com
© 2006 AlphaMed Press

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TISSUE-SPECIFIC STEM CELLS

Immunophenotype of Human Adipose-Derived Cells: Temporal Changes in Stromal-Associated and Stem Cell–Associated Markers

James B. Mitchella, Kevin McIntosha, Sanjin Zvonicb, Sara Garretta, Z. Elizabeth Floydb, Amy Klosterc, Yuan Di Halvorsenc,d, Robert W. Stormse, Brian Gohb, Gail Kilroyb, Xiying Wub, Jeffrey M. Gimbleb,c

a Cognate Therapeutics, Inc., Baltimore, Maryland;
b Stem Cell Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana;
c Artecel Sciences, Durham, North Carolina;
d CuraGen Corporation, Branford, Connecticut;
e Duke University Medical Center, Durham, North Carolina, USA

Key Words. Adipocyte • Adipose-derived stem cells • Aldehyde dehydrogenase • Alkaline phosphatase • Bone • Colony-forming unit • Osteoblast • Stromal vascular fraction

Correspondence: Jeffrey M. Gimble, M.D., Ph.D., Stem Cell Laboratory, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USA. Telephone: 225-763-3171; fax: 225-763-0273; e-mail: gimblejm{at}pbrc.edu

Adipose tissue represents an abundant and accessible source of multipotent adult stem cells and is used by many investigators for tissue engineering applications; however, not all laboratories use cells at equivalent stages of isolation and passage. We have compared the immunophenotype of freshly isolated human adipose tissue-derived stromal vascular fraction (SVF) cells relative to serial-passaged adipose-derived stem cells (ASCs). The initial SVF cells contained colony-forming unit fibroblasts at a frequency of 1:32. Colony-forming unit adipocytes and osteoblasts were present in the SVF cells at comparable frequencies (1:28 and 1:16, respectively). The immunophenotype of the adipose-derived cells based on flow cytometry changed progressively with adherence and passage. Stromal cell–associated markers (CD13, CD29, CD44, CD63, CD73, CD90, CD166) were initially low on SVF cells and increased significantly with successive passages. The stem cell–associated marker CD34 was at peak levels in the SVF cells and/or early-passage ASCs and remained present, although at reduced levels, throughout the culture period. Aldehyde dehydrogenase and the multidrug-resistance transport protein (ABCG2), both of which have been used to identify and characterize hematopoietic stem cells, are expressed by SVF cells and ASCs at detectable levels. Endothelial cell–associated markers (CD31, CD144 or VE-cadherin, vascular endothelial growth factor receptor 2, von Willebrand factor) were expressed on SVF cells and did not change significantly with serial passage. Thus, the adherence to plastic and subsequent expansion of human adipose-derived cells in fetal bovine serum-supplemented medium selects for a relatively homogeneous cell population, enriching for cells expressing a stromal immunophenotype, compared with the heterogeneity of the crude SVF.




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