HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 2005-0185v1 24/3/631    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jaatinen, T. Articles by Partanen, J. Search for Related Content PubMed PubMed Citation Articles by Jaatinen, T. Articles by Partanen, J.

STEM CELL GENETICS AND GENOMICS

Global Gene Expression Profile of Human Cord Blood–Derived CD133⁺ Cells

^a Research and Development and
^b Department of Tissue Typing, Finnish Red Cross Blood Service, Helsinki, Finland;
^c Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA;
^d Institute of Signal Processing, Tampere University of Technology, Tampere, Finland

Key Words. Human cord blood • Hematopoietic stem cells • Microarray

Correspondence: Jukka Partanen, Ph.D., Department of Tissue Typing, Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland. Telephone: +358-9-5801298; Fax: +358-9-5801495; e-mail: jukka.partanen{at}bts.redcross.fi

Received April 22, 2005; accepted for publication September 28, 2005.
Human cord blood (CB)–derived CD133⁺ cells carry characteristics of primitive hematopoietic cells and proffer an alternative for CD34⁺ cells in hematopoietic stem cell (HSC) transplantation. To characterize the CD133⁺ cell population on a genetic level, a global expression analysis of CD133⁺ cells was performed using oligonucleotide microarrays. CD133⁺ cells were purified from four fresh CB units by immunomagnetic selection. All four CD133⁺ samples showed significant similarity in their gene expression pattern, whereas they differed clearly from the CD133⁺ control samples. In all, 690 transcripts were differentially expressed between CD133⁺ and CD133⁺ cells. Of these, 393 were increased and 297 were decreased in CD133⁺ cells. The highest overexpression was noted in genes associated with metabolism, cellular physiological processes, cell communication, and development. A set of 257 transcripts expressed solely in the CD133⁺ cell population was identified. Colony-forming unit (CFU) assay was used to detect the clonal progeny of precursors present in the studied cell populations. The results demonstrate that CD133⁺ cells express primitive markers and possess clonogenic progenitor capacity. This study provides a gene expression profile for human CD133⁺ cells. It presents a set of genes that may be used to unravel the properties of the CD133⁺ cell population, assumed to be highly enriched in HSCs.

This article has been cited by other articles:

				C. Langer, M. D. Radmacher, A. S. Ruppert, S. P. Whitman, P. Paschka, K. Mrozek, C. D. Baldus, T. Vukosavljevic, C.-G. Liu, M. E. Ross, et al. High BAALC expression associates with other molecular prognostic markers, poor outcome, and a distinct gene-expression signature in cytogenetically normal patients younger than 60 years with acute myeloid leukemia: a Cancer and Leukemia Group B (CALGB) study Blood, June 1, 2008; 111(11): 5371 - 5379. [Abstract] [Full Text] [PDF]

				L. G. Spector, A. J. Hooten, and J. A. Ross Ontogeny of Gene Expression: A Changing Environment for Malignancy Cancer Epidemiol. Biomarkers Prev., May 1, 2008; 17(5): 1021 - 1023. [Full Text] [PDF]

				T.-S. Huang, J.-Y. Hsieh, Y.-H. Wu, C.-H. Jen, Y.-H. Tsuang, S.-H. Chiou, J. Partanen, H. Anderson, T. Jaatinen, Y.-H. Yu, et al. Functional Network Reconstruction Reveals Somatic Stemness Genetic Maps and Dedifferentiation-Like Transcriptome Reprogramming Induced by GATA2 Stem Cells, May 1, 2008; 26(5): 1186 - 1201. [Abstract] [Full Text] [PDF]

				M.-S. Tsai, S.-M. Hwang, K.-D. Chen, Y.-S. Lee, L.-W. Hsu, Y.-J. Chang, C.-N. Wang, H.-H. Peng, Y.-L. Chang, A.-S. Chao, et al. Functional Network Analysis of the Transcriptomes of Mesenchymal Stem Cells Derived from Amniotic Fluid, Amniotic Membrane, Cord Blood, and Bone Marrow Stem Cells, October 1, 2007; 25(10): 2511 - 2523. [Abstract] [Full Text] [PDF]

				E. Martin-Rendon, S. J.M. Hale, D. Ryan, D. Baban, S. P. Forde, M. Roubelakis, D. Sweeney, M. Moukayed, A. L. Harris, K. Davies, et al. Transcriptional Profiling of Human Cord Blood CD133+ and Cultured Bone Marrow Mesenchymal Stem Cells in Response to Hypoxia Stem Cells, April 1, 2007; 25(4): 1003 - 1012. [Abstract] [Full Text] [PDF]