| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
a Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas, USA;
b ViaCell Singapore Research Centre, Singapore;
c Laboratory of Neuroscience, National Institute on Aging, Bethesda, Maryland, USA;
d Children’s Hospital, Galveston, Texas, USA
Key Words. Wharton’s jelly • Flow cytometry • Regenerative medicine • In vitro expansion • Noncontroversial source of stem cells
Correspondence: Mark L. Weiss, Ph.D., Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506, USA. Telephone: 785-532-4520; Fax: 785-532-4557; e-mail: weiss{at}vet.ksu.edu
Received on July 20, 2005;
accepted for publication on October 2, 2005.
The umbilical cord contains an inexhaustible, noncontroversial source of stem cells for therapy. In the U.S., stem cells found in the umbilical cord are routinely placed into bio-hazardous waste after birth. Here, stem cells derived from human umbilical cord Wharton’s Jelly, called umbilical cord matrix stem (UCMS) cells, are characterized. UCMS cells have several properties that make them of interest as a source of cells for therapeutic use. For example, they 1) can be isolated in large numbers, 2) are negative for CD34 and CD45, 3) grow robustly and can be frozen/thawed, 4) can be clonally expanded, and 5) can easily be engineered to express exogenous proteins. UCMS cells have genetic and surface markers of mesenchymal stem cells (positive for CD10, CD13, CD29, CD44, and CD90 and negative for CD14, CD33, CD56, CD31, CD34, CD45, and HLA-DR) and appear to be stable in terms of their surface marker expression in early passage (passages 4–8). Unlike traditional mesenchymal stem cells derived from adult bone marrow stromal cells, small populations of UCMS cells express endoglin (SH2, CD105) and CD49e at passage 8. UCMS cells express growth factors and angiogenic factors, suggesting that they may be used to treat neurodegenerative disease. To test the therapeutic value of UCMS cells, undifferentiated human UCMS cells were transplanted into the brains of hemiparkinsonian rats that were not immune-suppressed. UCMS cells ameliorated apomorphine-induced rotations in the pilot test. UCMS cells transplanted into normal rats did not produce brain tumors, rotational behavior, or a frank host immune rejection response. In summary, the umbilical cord matrix appears to be a rich, noncontroversial, and inexhaustible source of primitive mesenchymal stem cells.
This article has been cited by other articles:
|
|
 |
|
  Y. Moodley, D. Atienza, U. Manuelpillai, C. S. Samuel, J. Tchongue, S. Ilancheran, R. Boyd, and A. Trounson Human Umbilical Cord Mesenchymal Stem Cells Reduce Fibrosis of Bleomycin-Induced Lung Injury Am. J. Pathol., July 1, 2009; 175(1): 303 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Weiss, C. Anderson, S. Medicetty, K. B. Seshareddy, R. J. Weiss, I. VanderWerff, D. Troyer, and K. R. McIntosh Immune Properties of Human Umbilical Cord Wharton's Jelly-Derived Cells Stem Cells, November 1, 2008; 26(11): 2865 - 2874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Troyer and M. L. Weiss Concise Review: Wharton's Jelly-Derived Cells Are a Primitive Stromal Cell Population Stem Cells, March 1, 2008; 26(3): 591 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Karahuseyinoglu, C. Kocaefe, D. Balci, E. Erdemli, and A. Can Functional Structure of Adipocytes Differentiated from Human Umbilical Cord Stroma-Derived Stem Cells Stem Cells, March 1, 2008; 26(3): 682 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Jones and D. McGonagle Human bone marrow mesenchymal stem cells in vivo Rheumatology, February 1, 2008; 47(2): 126 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Secco, E. Zucconi, N. M. Vieira, L. L.Q. Fogaca, A. Cerqueira, M. D. F. Carvalho, T. Jazedje, O. K. Okamoto, A. R. Muotri, and M. Zatz Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood! Stem Cells, January 1, 2008; 26(1): 146 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Can and S. Karahuseyinoglu Concise Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem Cells, November 1, 2007; 25(11): 2886 - 2895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Park, K. H. Jung, S. H. Kim, K. S. Kim, M. R. Choi, Y. Kim, and Y. G. Chai Functional Expression of Ion Channels in Mesenchymal Stem Cells Derived from Umbilical Cord Vein Stem Cells, August 1, 2007; 25(8): 2044 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Baksh, R. Yao, and R. S. Tuan Comparison of Proliferative and Multilineage Differentiation Potential of Human Mesenchymal Stem Cells Derived from Umbilical Cord and Bone Marrow Stem Cells, June 1, 2007; 25(6): 1384 - 1392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Lund, S. Wang, B. Lu, S. Girman, T. Holmes, Y. Sauve, D. J. Messina, I. R. Harris, A. J. Kihm, A. M. Harmon, et al. Cells Isolated from Umbilical Cord Tissue Rescue Photoreceptors and Visual Functions in a Rodent Model of Retinal Disease Stem Cells, March 1, 2007; 25(3): 602 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Karahuseyinoglu, O. Cinar, E. Kilic, F. Kara, G. G. Akay, D. O. Demiralp, A. Tukun, D. Uckan, and A. Can Biology of Stem Cells in Human Umbilical Cord Stroma: In Situ and In Vitro Surveys Stem Cells, February 1, 2007; 25(2): 319 - 331. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
